Characterization of a basic phospholipase A2-homologeu myotoxin isolated from the venom of the snake Bothrops neuwiedii (yarará chica) from Argentina

dc.creatorGeoghegan, Patricia A.
dc.creatorAngulo, Yamileth
dc.creatorCangelosi, Adriana
dc.creatorDíaz, Mónica
dc.creatorLomonte, Bruno
dc.date2020-09-17T01:20:54Z
dc.date2020-09-17T01:20:54Z
dc.date1999-12
dc.date.accessioned2023-08-29T20:06:59Z
dc.date.available2023-08-29T20:06:59Z
dc.identifier0041-0101
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1654
dc.identifier10.1016/s0041-0101(99)00115-4
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519426
dc.descriptionFil: Geoghegan, Patricia A. ANLIS Dr.C.G.Malbrán. Centro Nacional de Control de Calidad de Biológicos. Servicio de Inmunoterapéuticos; Argentina.
dc.descriptionFil: Angulo, Yamileth. Universidad de Costa Rica. Facultad de Microbiología. Instituto Clodomiro Picado; Costa Rica.
dc.descriptionFil: Cangelosi, Adriana. ANLIS Dr.C.G.Malbrán. Centro Nacional de Control de Calidad de Biológicos. Servicio de Inmunoterapéuticos; Argentina.
dc.descriptionFil: Díaz, Mónica. ANLIS Dr.C.G.Malbrán. Centro Nacional de Control de Calidad de Biológicos. Servicio de Inmunoterapéuticos; Argentina.
dc.descriptionFil: Lomonte, Bruno. Universidad de Costa Rica. Facultad de Microbiología. Instituto Clodomiro Picado; Costa Rica.
dc.descriptionA basic protein was isolated by CM-Sephadex C-25 chromatography from the venom of Bothrops neuwiedii from Argentina, and named B. neuwiedii myotoxin I. This protein exerted local myotoxic and edema-forming effects in mice, with potencies comparable to other myotoxins isolated from Bothrops spp. venoms. When injected by i.v. route at doses up to 4.7 mg/kg of body weight, the toxin was not lethal. In vitro, the toxin had no detectable phospholipase A2 activity on egg yolk phospholipids. B. neuwiedii myotoxin I appeared as a homodimer in sodium dodecylsulphate-polyacrylamide gel electrophoresis, with a subunit molecular weight of 15 kD. Gel immunodiffusion revealed a pattern of partial antigenic identity between the newly isolated myotoxin and myotoxin II from Bothrops asper venom. The sequence of B. neuwiedii myotoxin I was determined for the first 40 amino acid residues, showing high homology to several class II phospholipase A2 myotoxins of the Lys-49 family from crotalids. Altogether, results suggest that this toxin is a new member of the Lys-49 phospholipase A2-homologues with myotoxic, cytolytic, and edema-inducing activities.
dc.languageen
dc.relationToxicon : official journal of the International Society on Toxinology
dc.rightsnone
dc.subjectVenenos de Serpiente
dc.subjectArgentina
dc.subjectFosfolipasas A2
dc.subjectEdema
dc.subjectBothrops
dc.titleCharacterization of a basic phospholipase A2-homologeu myotoxin isolated from the venom of the snake Bothrops neuwiedii (yarará chica) from Argentina
dc.typeArtículo


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