Isolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patient

dc.creatorMartino, Florencia
dc.creatorTijet, Nathalie
dc.creatorMelano, Roberto
dc.creatorPetroni, Alejandro
dc.creatorHeinz, E
dc.creatorDe Belder, Denise
dc.creatorFaccone, Diego
dc.creatorRapoport, Melina J.
dc.creatorBiondi, Estefania
dc.creatorRodrigo, Veronica
dc.creatorVázquez, Miryam
dc.creatorPasteran, Fernando
dc.creatorThomson, Nicholas R
dc.creatorCorso, Alejandra
dc.creatorGomez, Sonia A.
dc.date2020-03-27T20:06:49Z
dc.date2020-03-27T20:06:49Z
dc.date2019-08-09
dc.date.accessioned2023-08-29T20:06:44Z
dc.date.available2023-08-29T20:06:44Z
dc.identifier1932-6203
dc.identifierhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0221960
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1531
dc.identifier10.1371/journal.pone.0221960
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519285
dc.descriptionFil: Martino, Florencia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentin
dc.descriptionFil: Tijet, Nathalie. Public Health Ontario Laboratories, Toronto, Ontario; Canadá.
dc.descriptionFil: Melano, Roberto. Public Health Ontario Laboratories, Toronto, Ontario; Canadá.
dc.descriptionFil: Petroni, Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Heinz, E. The Welcome Trust Sanger Institute, Hinxton, Cambridge; Reino Unido.
dc.descriptionFil: De Belder, Denise. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Faccone, Diego. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Rapoport, Melina J. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Biondi, Estefania. Hospital de Niños Dr. Ricardo Gutiérrez, Ciudad Autónoma de Buenos Aires; Argentina.
dc.descriptionFil: Rodrigo, Veronica. Hospital de Niños Dr. Ricardo Gutiérrez, Ciudad Autónoma de Buenos Aires; Argentina.
dc.descriptionFil: Vázquez, Miryam. Hospital de Niños Dr. Ricardo Gutiérrez, Ciudad Autónoma de Buenos Aires; Argentina.
dc.descriptionFil: Pasteran, Fernando. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Thomson, Nicholas R. The Welcome Trust Sanger Institute, Hinxton, Cambridge; Reino Unido.
dc.descriptionFil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Gomez, Sonia A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionIn Argentina, NDM metallo-β-lactamase was first reported in 2013. By now, it has disseminated throughout the country in diverse Gram negative bacteria. Here, we report the case of a paediatric patient that underwent a 1-year hospitalisation due to erythrodermic psoriasis in 2014 and received multiple antimicrobial treatments. During his stay, five isolates were obtained from rectal swabs (rs) or blood culture (bc) suspicious of carbapenemase production: a K. quasipneumoniae subsp. quasipneumoniae (rs), Citrobacter freundii (rs), Escherichia coli (bc), Enterobacter cloacae (rs), and a Serratia marcescens (bc). The isolates were studied with broth microdilution, biparental conjugation and plasmid and whole genome sequencing (Illumina). All isolates harboured an 138,998-bp type 1 IncC plasmid that carried blaNDM-1, bleMBL, blaCMY-6, rmtC, aac(6')-Ib, and sul1 resistance genes. Additionally, the blaNDM-plasmids contained ISKpn8 an insertion sequence previously described as associated only to blaKPC. One isolate, a colistin-resistant E. coli, also carried a mcr-1-containing an IncI2 plasmid, which did not harbour additional resistance. The whole genome of K. quasipneumoniae subsp. quasipneumoniae isolate was fully sequenced. This isolate harboured, additionally to blaNDM, three plasmid-mediated quinolone resistance genes: qnrB4, qnrB52 and aac(6')-Ib-cr1. The E. cloacae isolate also harboured qnrA1. These findings alert to the underestimated horizontal dissemination of multidrug-resistant plasmids limiting treatment options with last resort antimicrobials. This research was funded by Agencia Nacional de Promoción Científica y Tecnológica - Fondo para la Investigación Científica y Tecnológica, PICT-2012-0145 to SAG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.formatpdf
dc.languageen
dc.relationPloS one
dc.rightsopen
dc.sourcePLoS One 2019;14(9):e0221960
dc.subjectPediatría
dc.subjectEnterobacteriaceae
dc.subjectPlásmidos
dc.titleIsolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patient
dc.typeArtículo


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