Echinococcus granulosus: DNA extraction from germinal layers allows strain determination in fertile and nonfertile hydatid cysts

dc.creatorKamenetzky, Laura
dc.creatorCanova, S G
dc.creatorGuarnera, Eduardo
dc.creatorRosenzvit, Mara C.
dc.date2019-12-12T20:36:37Z
dc.date2019-12-12T20:36:37Z
dc.date2000-06
dc.date.accessioned2023-08-29T20:06:40Z
dc.date.available2023-08-29T20:06:40Z
dc.identifier0014-4894
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1503
dc.identifier10.1006/expr.2000.4518
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519244
dc.descriptionA method for the isolation of Echinococcus granulosus DNA from germinal layers of hydatid cysts is described. The method includes a hexadecyltrimethylammonium bromide/chloroform extraction and an adsorption to diatomaceous earth suspension. DNA suitable for polymerase chain reaction was obtained and used for parasite strain determination by mitochondrial cytochrome c oxidase I gene sequencing. Fertile and nonfertile cyst isolates from sheep, cattle, pigs, and humans were characterized. Hitherto, no direct parasite strain characterization has been made on nonfertile hydatid cysts, whereas here we report that nonfertile hydatid cysts were produced by sheep strain (G1 genotype) in sheep, cattle, and humans and by pig strain (G7 genotype) in pigs.
dc.languageen
dc.relationExperimental parasitology
dc.rightsnone
dc.titleEchinococcus granulosus: DNA extraction from germinal layers allows strain determination in fertile and nonfertile hydatid cysts
dc.typeArtículo


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