PCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNA

dc.creatorHong, Xiao-Kun
dc.creatorZhang, Xuan
dc.creatorFusco, Octavio Alejandro
dc.creatorLan, You-Gen
dc.creatorLun, Zhao-Rong
dc.creatorLai, De-Hua
dc.date2019-12-04T19:53:34Z
dc.date2019-12-04T19:53:34Z
dc.date2017-07
dc.date.accessioned2023-08-29T20:06:25Z
dc.date.available2023-08-29T20:06:25Z
dc.identifier1873-6254
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1440
dc.identifier10.1016/j.actatropica.2017.04.007
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519139
dc.descriptionFil: Hong, Xiao-Kun. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
dc.descriptionFil: Zhang, Xuan. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
dc.descriptionFil: Fusco, Octavio Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
dc.descriptionFil: Lan, You-Gen. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
dc.descriptionFil: Lun, Zhao-Rong. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
dc.descriptionFil: Lai, De-Hua. Sun Yat-Sen University. School of Life Sciences. State Key Laboratory of Biocontrol. Center for Parasitic Organisms, Cantón; China.
dc.descriptionTrypanosoma lewisi, transmitted by rat fleas, is a widespread pathogen specific to rats with records of human infection cases. Its closely related species with global distribution, Trypanosoma musculi, is transmitted between mice by ingestion of infected fleas. These trypanosomes are of similar morphology, making it difficult to distinguish them by microscopy. In this study, we have developed a rapid, sensitive and reliable PCR method for the diagnosis of T. lewisi and T. musculi. The T. lewisi-specific amplicons were not produced by other Trypanosoma, such as T. musculi, T. brucei complex or T. cruzi, neither by an outgroup of Leishmania amazonensis. The detection limits of the three pairs of T. lewisi-specific primers were 50ng, 1ng and 10ng of total DNA, respectively. The primers designed for T. musculi primers showed specifically that amplicon strictly in T. musculi and their detection limits were 10ng and 1ng of total DNA. To simplify the detection process, we managed to apply our method directly on tail blood samples without complicated DNA purification. In conclusion, PCR with our primers could be a highly sensitive, specific protocol to detect and distinguish T. lewisi and T. musculi from other trypanosomes.
dc.formatpdf
dc.languageen
dc.relationActa tropica
dc.rightsnone
dc.sourceActa Trópica 2017;171:207-212
dc.subjectADN
dc.subjectTrypanosoma
dc.subjectGeomorfología
dc.titlePCR-based identification of Trypanosoma lewisi and Trypanosoma musculi using maxicircle kinetoplast DNA
dc.typeArtículo


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