An rK28-Based Immunoenzymatic Assay for the Diagnosis of Canine Visceral Leishmaniasis in Latin America

dc.creatorLauricella, Marta A.
dc.creatorMaidana, Cristina Graciela
dc.creatorFrias, Victoria Fragueiro
dc.creatorRomagosa, Carlo M
dc.creatorNegri, Vanesa
dc.creatorBenedetti, Ruben
dc.creatorSinagra, Angel
dc.creatorLuna, María Concepción
dc.creatorTartaglino, Lilian
dc.creatorLaucella, Susana A.
dc.creatorReed, Steven G
dc.creatorRiarte, Adelina
dc.date2019-12-04T19:24:26Z
dc.date2019-12-04T19:24:26Z
dc.date2016
dc.date.accessioned2023-08-29T20:06:23Z
dc.date.available2023-08-29T20:06:23Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1433
dc.identifierhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944717/
dc.identifier10.4269/ajtmh.13-0768
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519125
dc.descriptionFil: Lauricella, Marta Alicia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; Argentina.
dc.descriptionFil: Maidana, Cristina Graciela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; Argentina.
dc.descriptionFil: Frias, Victoria Fragueiro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; Argentina.
dc.descriptionFil: Romagosa, Carlo M. Municipalidad de Posadas. Secretaría de Calidad de Vida; Misiones, Argentina.
dc.descriptionFil: Negri, Vanesa. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; Argentina.
dc.descriptionFil: Tartaglino, Lilian. Municipalidad de Posadas. Secretaría de Calidad de Vida; Misiones, Argentina.
dc.descriptionFil: Laucella, Susana A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; Argentina.
dc.descriptionFil: Reed, Steven G. Infectious Disease Research Institute; Washington, Estados Unidos.
dc.descriptionFil: Riarte, Adelina R. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; Argentina.
dc.descriptionDirect observation of Leishmania parasites in tissue aspirates has shown low sensitivity for the detection of canine visceral leishmaniasis (VL). Therefore in the last quarter century immunoenzymatic tests have been developed to improve diagnosis. The purpose of this study was to develop a fast recombinant K28 antigen, naked-eye qualitative enzyme-linked immunosorbent assay (VL Ql-ELISA) and a quantitative version (VL Qt-ELISA), and to display it in a kit format, whose cutoff value (0.156) was selected as the most adequate one to differentiate reactive from nonreactive samples. Considering 167 cases and 300 controls, sensitivity was 91% for both assays and specificity was 100% and 98.7% in Ql-ELISA and Qt-ELISA, respectively. Positive predictive values were 100% and 97.4% for Ql-ELISA and Qt-ELISA, respectively, and negative predictive values were 95.2% for both ELISAs. Reagent stability, reliability studies, including periodic repetitions and retest of samples, cutoff selection, and comparison of rK28 ELISAs with rK39 immunochromatographic test, were the international criteria that supported the quality in both kits. The performance of both ELISA kits in this work confirmed their validity and emphasized their usefulness for low-to-medium complexity laboratories.
dc.formatpdf
dc.languageen
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationThe American journal of tropical medicine and hygiene
dc.rightsopen
dc.subjectEnfermedad de Chagas
dc.subjectLeishmaniasis Visceral
dc.subjectTécnicas para Inmunoenzimas
dc.subjectAnticuerpos Antiprotozoarios
dc.subjectAntígenos de Protozoos
dc.titleAn rK28-Based Immunoenzymatic Assay for the Diagnosis of Canine Visceral Leishmaniasis in Latin America
dc.typeArtículo


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