Multimethodological Approach to Gastrointestinal Microsporidiosis in HIV-Infected Patients

dc.creatorVelásquez, Jorge Néstor
dc.creatordi Risio, Cecilia
dc.creatorEtchart, Cristina
dc.creatorChertcoff, Agustín Víctor
dc.creatorAstudillo, Osvaldo Germán
dc.creatorCarnevale, Silvana
dc.date2019-11-20T19:56:37Z
dc.date2019-11-20T19:56:37Z
dc.date2019-07-08
dc.date.accessioned2023-08-29T20:05:59Z
dc.date.available2023-08-29T20:05:59Z
dc.identifier1896-1851
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1309
dc.identifier10.2478/s11686-019-00095-z
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8518918
dc.descriptionFil: Velásquez,Jorge Néstor. Hospital de Infecciosas Francisco Javier Muñiz, Ciudad Autónoma de Buenos Aires; Argentina.
dc.descriptionFil: di Risio, Cecilia. Hospital General de Agudos J. M. Penna, Ciudad Autónoma de Buenos Aires; Argentina. ,
dc.descriptionFil: Etchart, Cristina. Hospital General de Agudos J. M. Penna, Ciudad Autónoma de Buenos Aires; Argentina.
dc.descriptionFil: Chertcoff, Agustín Víctor. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Astudillo, Osvaldo Germán. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionPurpose Microsporidiosis is an opportunistic infection that produces chronic diarrhoea and cholangiopathy in patients with AIDS, mainly caused by two species of microsporidia, Enterocytozoon bieneusi and Encephalitozon intestinalis. The aim of this work was to develop an integral system for the diagnosis of microsporidiosis of the intestine and biliary tract in HIV-infected patients, comprising microscopic and molecular techniques. Methods The study population comprised 143 adult patients of both sexes with diagnosis of HIV infection, with chronic diarrhoea, and with or without HIV-associated cholangiopathy. Stool studies for microsporidia identification of spores were performed on each patient. A video esofagogastroduodenoscopy with biopsy collection was also carried out for routine histology and semi-thin sections stained with Azure II. Species identification was carried out by transmission electron microscopy and/or polymerase chain reaction for the species E. bieneusi and E. intestinalis. Results Out of the 143 patients a total of 12.6% (n = 18) were infected with microsporidia. Microsporidia species identified in most cases was E. bieneusi (16/18 cases), followed by E. intestinalis (4/18), all of these last ones in coinfection with E. bieneusi. Conclusions Clinical, imaging, microscopic and molecular analyses, when applied in a systematic and integrated approach, allow diagnosis and identification of microsporidia at species level in AIDS patients with chronic diarrhoea, and with or without HIV-associated cholangiopathy.
dc.formatpdf
dc.languageen
dc.relationActa parasitologica
dc.rightsnone
dc.sourceActa Parasitologica 2019;64:658-669
dc.subjectEncephalitozoon
dc.subjectMicrosporidiosis
dc.subjectVIH
dc.titleMultimethodological Approach to Gastrointestinal Microsporidiosis in HIV-Infected Patients
dc.typeArtículo


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