An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study

dc.creatorRubio Carrasquilla, Marcela
dc.creatorSanta Escobar, Cristian David
dc.creatorRondón Marín, Juan Pablo
dc.creatorBotero Garcés, Jorge Humberto
dc.creatorGuimaraes, Allan J.
dc.creatorMoreno Frias, Ernesto
dc.creatorCano Restrepo, Luz Elena
dc.date2022-03-14T16:58:20Z
dc.date2022-03-14T16:58:20Z
dc.date2018
dc.date.accessioned2023-08-28T20:47:38Z
dc.date.available2023-08-28T20:47:38Z
dc.identifierRubio-Carrasquilla M, Santa CD, Rendón JP, Botero-Garcés J, Guimarães AJ, Moreno E, Cano LE. An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study. Med Mycol. 2019 Aug 1;57(6):724-732. doi: 10.1093/mmy/myy131. Rubio-Carrasquilla M, Santa CD, Rendón JP, Botero-Garcés J, Guimarães AJ, Moreno E, Cano LE. An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study. Med Mycol. 2019 Aug 1;57(6):724-732. doi: 10.1093/mmy/myy131.
dc.identifier1369-3786
dc.identifierhttp://hdl.handle.net/10495/26552
dc.identifier10.1093/mmy/myy131
dc.identifier1460-2709
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8484350
dc.descriptionABSTRACT : Histoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our knowledge, we explore the suitability of an interferon gamma release assay (IGRA) to detect latent Hc infection in asymptomatic individuals. A cohort of 126 volunteers was enrolled in the study, 13 of which underwent a Hc infection in the past, and 93 of them showing risk factors for this infection. The remaining 20 participants did not refer any risk factors of Hc infection, but eight of them showed evidences of infection with Mycobacterium tuberculosis. All participants were recruited in Medellin, Colombia, between January 2014 and December 2017. Whole blood samples were cultured with four different Hc crude antigens and phytohemaglutinin as positive control. The interferon (IFN)-γ released by T lymphocytes upon antigen stimulation was quantified by ELISA. A defined cutoff value of 20 pg/ml for the IFN-γ concentration allowed us to distinguish between the group with documented past infections and the group of noninfected individuals with high sensitivity (70– 92%) and specificity (85–95%), for the four tested antigens. Positive 82–95% and negative 77–92% predictive values were also very high, comparable to those reported for commercially available IGRAs. The new test constitutes a promising screening method to detect individuals with latent Hc infection, even decades after the primary infection, as evidenced in this study.
dc.descriptionCOL0013709
dc.descriptionCOL0007506
dc.format9
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherOxford University Press
dc.publisherGrupo de Parasitología Universidad de Antioquia
dc.publisherMicología Médica y Experimental
dc.publisherOxford, Inglaterra
dc.relationMed. Mycol.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectAntígenos Fúngicos
dc.subjectAntigens, Fungal
dc.subjectHistoplasmosis
dc.subjectHistoplasma
dc.subjectHistoplasmosis
dc.subjectEnsayos de Liberación de Interferón gamma
dc.subjectInterferon-gamma Release Tests
dc.subjectValor Predictivo de las Pruebas
dc.subjectPredictive Value of Tests
dc.subjectPrueba de Estudio Conceptual
dc.subjectProof of Concept Study
dc.titleAn interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/acceptedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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