Immunosuppression in Cervical Cancer with Special Reference to Arginase Activity

dc.creatorBedoya, Astrid Milena
dc.creatorTate, David
dc.creatorBaena Zapata, Armando
dc.creatorCórdoba Gómez, Carlos Mario
dc.creatorBorrero Franco, Mauricio
dc.creatorPareja, René
dc.creatorRojas, Fredy
dc.creatorPatterson, John
dc.creatorHerrero, Rolando
dc.creatorZea, Arnold
dc.creatorSánchez Vásquez, Gloria Inés
dc.date2022-11-29T21:46:35Z
dc.date2022-11-29T21:46:35Z
dc.date2014
dc.date.accessioned2023-08-28T20:07:58Z
dc.date.available2023-08-28T20:07:58Z
dc.identifier0090-8258
dc.identifierhttps://hdl.handle.net/10495/32451
dc.identifier10.1016/j.ygyno.2014.07.096
dc.identifier1095-6859
dc.identifierhttps://www.sciencedirect.com/science/article/pii/S0090825814012190?via%3Dihub
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8474359
dc.descriptionABSTRACT: Introduction: Cervical cancer is characterized by an immunosuppressive microenvironment and a Th2-type cytokine profile. Expression of arginase (ASE), the enzyme that converts L-arginine into L-ornithine and urea, is stimulated by Th2-type cytokines. Objective: To assess the association of ASE activity and L-Arg metabolism products with cervical cancer. Methods: Sera of 87 and 41 women with histologically confirmed by colposcopy-directed biopsy SCC and CIN3 respectively and 79 with normal cytology or Low-Grade Squamous Intraepithelial Lesion (LSIL), were evaluated. Cytokines were measured using Milliplex Human cytokine/chemokine kit. Arginase (ASE) activity was determined using an enzymatic assay. Levels of L-arginine, L ornithine, putrescine and spermine were determined by HPLC. Results: Significantly higher levels of ASE activity were observed in women with CIN3 (age-adjusted OR: 24.3; 95%CI: 3.82–155) and SCC (AOR: 9.8; 95%CI: 2.34–40.8). As expected, possibly due to high levels of ASE activity, higher levels of L-Arg were negatively associated with CIN3 (AOR: 0.03; 95%CI: 0.004–0.19) and SSC (AOR: 0.06; 95%CI: 0.02–0.24). Consistent with the role of ASE in the conversion of L-arginine to L-ornithine and polyamine production therefrom, women with cervical cancer had higher levels of spermine and putrescine. A correlation analysis revealed a significant albeit weak relationship between high levels of IL-10 and high levels of ASE (Pearson r = 0.32, p-value = 0.003) in women with cervical cancer. Conclusion: This study indicates that ASE activity and L-Arg degradation mechanisms of immunosuppression are present in cervical cancer. The results foster research in the design of possible strategies to inhibit ASE activity for therapy of cervical cancer.
dc.descriptionCOL0012328
dc.format7
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.publisherInfección y Cáncer
dc.publisherNueva York, Estados Unidos
dc.relationGynecol. Oncol.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectUterine Cervical Neoplasms
dc.subjectNeoplasias del Cuello Uterino
dc.subjectArginase
dc.subjectArginasa
dc.subjectArginine
dc.subjectArginina
dc.subjectCytokines
dc.subjectCitocinas
dc.subjectTh1-Th2 Balance
dc.subjectBalance Th1 - Th2
dc.subjectColombia
dc.titleImmunosuppression in Cervical Cancer with Special Reference to Arginase Activity
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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