dc.creatorUsme Ciro, José Aldemar
dc.creatorLopera Madrid, Jaime Alberto
dc.creatorÁlvarez Díaz, Diego Alejandro
dc.creatorEnjuanes, Luis
dc.creatorAlmazán, Fernando
dc.date2021-10-19T21:35:17Z
dc.date2021-10-19T21:35:17Z
dc.date2016
dc.date.accessioned2023-08-28T19:56:54Z
dc.date.available2023-08-28T19:56:54Z
dc.identifier0300-5526
dc.identifierhttp://hdl.handle.net/10495/23269
dc.identifier10.1159/000476066
dc.identifier1423-0100
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8471372
dc.descriptionABSTRACT: Dengue viruses (DENV) have become the most important arthropod-borne viruses, causing dengue and severe dengue fever in at least 50–100 million cases each year, mainlyin tropical and subtropical countries. During recent years, important advances in the molecular biology concerning the life cycle of these viruses have allowed the manipulation and generation of recombinant viruses and replicons with multiple applications, mainly in viral biology and the screening of antiviral compounds. In the present study, we describe the construction of an enhanced green fluorescent protein- bearing DENV replicon under the control of the cytomegalo-virus immediate early promoter. Following a rational in silico design and cloning by standard molecular biology techniques, a reporter DENV-2 replicon and a replication-deficient mutant were constructed, and characterized by confocal microscopy and real-time RT-PCR. The results showed successful transcription, translation, and autonomous viral RNA replication of the DENV replicon from its DNA clone.
dc.descriptionCOL0140139
dc.format8
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherKarger
dc.publisherGrupo Medicina Molecular y de Translación
dc.publisherBasilea, Suiza
dc.relationIntervirology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectVirus del Dengue
dc.subjectDengue Virus
dc.subjectReplicón
dc.subjectReplicon
dc.subjectFlavivirus
dc.titleGeneration of a DNA-Launched Reporter Replicon Based on Dengue Virus Type 2 as a Multipurpose Platform
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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