CC8 MRSA strains harboring SCCmec Type IVc are predominant in Colombian hospitals

dc.creatorJiménez Quiceno, Judy Natalia
dc.creatorOcampo Ríos, Ana María
dc.creatorVanegas Múnera, Johanna Marcela
dc.creatorRodríguez Tamayo, Erika Andrea
dc.creatorMediavilla, José
dc.creatorChen, Liang
dc.creatorMuskus López, Carlos Enrique
dc.creatorVélez Agustín, Lázaro Giraldo
dc.creatorRojas Arbelaéz, Carlos Alberto
dc.creatorRestrepo Gouzy, Andrea
dc.creatorOspina, Sigifredo
dc.creatorGarcés, Carlos
dc.creatorFranco, Liliana
dc.creatorBifani, Pablo
dc.creatorKreiswirth, Barry
dc.creatorCorrea Ochoa, Margarita María
dc.date2021-09-02T15:29:35Z
dc.date2021-09-02T15:29:35Z
dc.date2012
dc.date.accessioned2023-08-28T19:56:47Z
dc.date.available2023-08-28T19:56:47Z
dc.identifier1932-6203
dc.identifierhttp://hdl.handle.net/10495/22063
dc.identifier10.1371/journal.pone.0038576
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8471340
dc.descriptionABSTRACT: Background: Recent reports highlight the incursion of community-associated MRSA within healthcare settings. However, knowledge of this phenomenon remains limited in Latin America. The aim of this study was to evaluate the molecular epidemiology of MRSA in three tertiary-care hospitals in Medellı ́n, Colombia. Methods: An observational cross-sectional study was conducted from 2008–2010. MRSA infections were classified as either community-associated (CA-MRSA) or healthcare-associated (HA-MRSA), with HA-MRSA further classified as hospital-onset (HAHO-MRSA) or community-onset (HACO-MRSA) according to standard epidemiological definitions established by the U.S. Centers for Disease Control and Prevention (CDC). Genotypic analysis included SCCmec typing, spa typing, PFGE and MLST. Results: Out of 538 total MRSA isolates, 68 (12.6%) were defined as CA-MRSA, 243 (45.2%) as HACO-MRSA and 227 (42.2%) as HAHO-MRSA. The majority harbored SCCmec type IVc (306, 58.7%), followed by SCCmec type I (174, 33.4%). The prevalence of type IVc among CA-, HACO- and HAHO-MRSA isolates was 92.4%, 65.1% and 43.6%, respectively. From 2008 to 2010, the prevalence of type IVc-bearing strains increased significantly, from 50.0% to 68.2% (p = 0.004). Strains harboring SCCmec IVc were mainly associated with spa types t1610, t008 and t024 (MLST clonal complex 8), while PFGE confirmed that the t008 and t1610 strains were closely related to the USA300-0114 CA-MRSA clone. Notably, strains belonging to these three spa types exhibited high levels of tetracycline resistance (45.9%). Conclusion: CC8 MRSA strains harboring SCCmec type IVc are becoming predominant in Medellı ́n hospitals, displacing previously reported CC5 HA-MRSA clones. Based on shared characteristics including SCCmec IVc, absence of the ACME element and tetracycline resistance, the USA300-related isolates in this study are most likely related to USA300-LV, the recently-described ‘Latin American variant’ of USA300.
dc.descriptionCOL0013746
dc.descriptionCOL0005744
dc.descriptionCOL0015099
dc.descriptionCOL0058784
dc.descriptionCOL0004362
dc.format10
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.publisherMicrobiología Molecular
dc.publisherGRIPE: Grupo Investigador de Problemas en Enfermedades Infecciosas
dc.publisherPrograma de Estudio y Control de Enfermedades Tropicales (PECET)
dc.publisherGrupo de Investigación Clínica en Enfermedades del Niño y del Adolescente - Pediaciencias
dc.publisherEpidemiología
dc.publisherSan Francisco, Estados Unidos
dc.relationPLoS ONE.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by/4.0/
dc.subjectStaphylococcus aureus
dc.subjectInfección Hospitalaria
dc.subjectCross Infection
dc.subjectEpidemiología Molecular
dc.subjectMolecular Epidemiology
dc.subjectResistencia a la Tetraciclina
dc.subjectTetracycline Resistance
dc.subjectStaphylococcus aureus resistente a meticilina (SARM)
dc.titleCC8 MRSA strains harboring SCCmec Type IVc are predominant in Colombian hospitals
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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