Validation and clinical application of a nested PCR for paracoccidioidomycosis diagnosis in clinical samples from Colombian patients

dc.creatorGaviria Camino, Marcela
dc.creatorRivera Arango, Vanessa
dc.creatorMuñoz Cadavid, Cesar
dc.creatorCano Restrepo, Luz Elena
dc.creatorNaranjo Preciado, Tonny Williams
dc.date2021-07-28T02:44:23Z
dc.date2021-07-28T02:44:23Z
dc.date2015
dc.date.accessioned2023-08-28T19:52:32Z
dc.date.available2023-08-28T19:52:32Z
dc.identifier1413-8670
dc.identifierhttp://hdl.handle.net/10495/21203
dc.identifier10.1016/j.bjid.2015.04.008
dc.identifier1678-4391
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8470070
dc.descriptionABSTRACT: Paracoccidioidomycosis is a systemic and endemic mycosis, restricted to tropical and sub-tropical areas of Latin America. The infection is caused by the thermal dimorphic fungusParacoccidioides brasiliensis and Paracoccidioides lutzii. The diagnosis of paracoccidioidomyco-sis is usually performed by microscopic examination, culture and immunodiagnostic teststo respiratory specimens, body fluids and/or biopsies; however these methods require labo-ratory personnel with experience and several days to produce a result. In the present study,we have validated and evaluated a nested PCR assay targeting the gene encoding the Para-coccidioides gp43 membrane protein in 191 clinical samples: 115 samples from patients withproven infections other than paracoccidioidomycosis, 51 samples as negative controls, and25 samples from patients diagnosed with paracoccidioidomycosis. Additionally, the speci-ficity of the nested PCR assay was also evaluated using purified DNA isolated from culturesof different microorganisms (n = 35) previously identified by culture and/or sequencing. Theresults showed that in our hands, this nested PCR assay for gp43 protein showed specificityand sensitivity rates of 100%. The optimized nested PCR conditions in our laboratory alloweddetection down to 1 fg of P. brasiliensis DNA.
dc.descriptionCOL0013709
dc.format8
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherBrazilian Society of Infectious Diseases
dc.publisherMicología Médica y Experimental
dc.publisherSalvador, Brasil
dc.relationBraz. J. Infect. Dis.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectParacoccidioidomicosis
dc.subjectParacoccidioidomycosis
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectPolymerase Chain Reaction
dc.subjectDiagnóstico molecular
dc.subjectParacoccidioides brasiliensis
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_34079
dc.titleValidation and clinical application of a nested PCR for paracoccidioidomycosis diagnosis in clinical samples from Colombian patients
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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