PCR with Paracoccidioides brasiliensis Specific Primers : Potential Use in Ecological Studies

dc.creatorDíez Posada, Soraya
dc.creatorPino Tamayo, Paula Andrea
dc.creatorCorredor Rengifo, Gabriel German
dc.creatorCastaño, John Harold
dc.creatorPeralta, Luis Alejandro
dc.creatorMcEwen Ochoa, Juan Guillermo
dc.creatorRestrepo Moreno, Ángela
dc.date2022-10-08T18:19:01Z
dc.date2022-10-08T18:19:01Z
dc.date1999
dc.date.accessioned2023-08-28T19:50:57Z
dc.date.available2023-08-28T19:50:57Z
dc.identifierDíez S, Garcia EA, Pino PA, Botero S, Corredor GG, Peralta LA, Castaño JH, Restrepo A, McEwen JG. PCR with Paracoccidioides brasiliensis specific primers: potential use in ecological studies. Rev Inst Med Trop Sao Paulo. 1999 Nov-Dec;41(6):351-8. doi: 10.1590/s0036-46651999000600004.
dc.identifier0036-4665
dc.identifierhttps://hdl.handle.net/10495/31149
dc.identifier10.1590/S0036-46651999000600004
dc.identifier1678-9946
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8469614
dc.descriptionABSTRACT: The precise microenvironment of Paracoccidioides brasiliensis has not yet been discovered perhaps because the methods used are not sensitive enough. We applied to this purpose the polymerase chain reaction (PCR) using three sets of specific primers corresponding to two P. brasiliensis genes. This fungus as well as several other fungi, were grown and their DNA obtained by mechanical disruption and a phenol chloroform isoamylalcohol-based purification method. The DNA served for a PCR reaction that employed specific primers from two P. brasiliensis genes that codify for antigenic proteins, namely, the 27 kDa and the 43 kDa. The lowest detection range for the 27 kDa gene was 3 pg. The amplification for both genes was positive only with DNA from P. brasiliensis; additionally, the mRNA for the 27 kDa gene was present only in P. brasiliensis, as indicated by the Northern analysis. The standardization of PCR technology permitted the amplification of P. brasiliensis DNA in artificially contaminated soils and in tissues of armadillos naturally infected with the fungus. These results indicate that PCR technology could play an important role in the search for P. brasiliensis’ habitat and could also be used in other ecological studies.
dc.descriptionCOL0013709
dc.format7
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherUniversidade de São Paulo, Faculdade de Medicina
dc.publisherMicología Médica y Experimental
dc.publisherSao Paulo, Brasil
dc.relationRev. Inst. Med. Trop. São Paulo.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectParacoccidioides
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectPolymerase Chain Reaction
dc.subjectCartilla de ADN
dc.subjectDNA Primers
dc.subjectNorthern Blotting
dc.subjectBlotting, Northern
dc.subjectADN de Hongos
dc.subjectDNA, Fungal
dc.titlePCR with Paracoccidioides brasiliensis Specific Primers : Potential Use in Ecological Studies
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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