Covalent Immobilization of Naringinase over TwoDimensional 2D Zeolites and its Applications in a Continuous Process to Produce Citrus Flavonoids and for Debittering of Juices

dc.creatorCarceller, Jose Miguel
dc.creatorMartínez Galán, Julián Paul
dc.creatorMonti, Rubens
dc.creatorBassan, Juliana Cristina
dc.creatorFilice, Marco
dc.creatorYu, Jihong
dc.creatorCliment, María J
dc.creatorIborra, Sara
dc.creatorCorma, Avelino
dc.date2022-01-16T13:37:42Z
dc.date2022-01-16T13:37:42Z
dc.date2020
dc.date.accessioned2023-08-28T19:49:43Z
dc.date.available2023-08-28T19:49:43Z
dc.identifier1867-3880
dc.identifierhttp://hdl.handle.net/10495/25318
dc.identifier10.1002/cctc.202000320
dc.identifier1867-3899
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8469249
dc.descriptionABSTRACT: The crude naringinase from Penicillium decumbens and a purified naringinase with high α‐L‐rhamnosidase activity could be covalently immobilized on two‐dimensional zeolite ITQ‐2 after surface modification with glutaraldehyde. The influence of pH and temperature on the enzyme activity (in free and immobilized forms) as well as the thermal stability were determined using the specific substrate: p‐nitrophenyl‐alpha‐L‐rhamnopyranoside (Rha‐pNP). The crude and purified naringinase supported on ITQ‐2 were applied in the hydrolysis of naringin, giving the flavonoids naringenin and prunin respectively with a conversion >90 % and excellent selectivity. The supported enzymes showed long term stability, being possible to perform up to 25 consecutive cycles without loss of activity, showing its high potential to produce the valuable citrus flavonoids prunin and naringenin. We have also succeeded in the application of the immobilized crude naringinase on ITQ‐2 for debittering grapefruit juices in a continuous process that was maintained operating for 300 h, with excellent results. [ABSTRACT FROM AUTHOR] Copyright of ChemCatChem is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
dc.descriptionCOL0000407
dc.format10
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley
dc.publisherAlimentación y Nutrición Humana
dc.publisherWeinheim, Alemania
dc.relationChemCatChem
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectITQ-2 zeolite
dc.subjectjuice citrus debittering
dc.subjectNaringenin
dc.subjectnaringin
dc.subjectprunin
dc.subjectsupported naringinase
dc.titleCovalent Immobilization of Naringinase over TwoDimensional 2D Zeolites and its Applications in a Continuous Process to Produce Citrus Flavonoids and for Debittering of Juices
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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