| dc.contributor | International Congress of Parasitology (15 : 21 de agosto de 2022 : Congress Venue – Bella Center, Copenhague, Dinamarca) | |
| dc.creator | Galván Díaz, Ana Luz | |
| dc.creator | García Montoya, Gisela María | |
| dc.creator | Alzate Restrepo, Juan Fernando | |
| dc.date | 2022-08-30T16:42:50Z | |
| dc.date | 2022-08-30T16:42:50Z | |
| dc.date | 2022-08-21 | |
| dc.date.accessioned | 2023-08-28T19:46:18Z | |
| dc.date.available | 2023-08-28T19:46:18Z | |
| dc.identifier | https://hdl.handle.net/10495/30310 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8468185 | |
| dc.description | ABSTRACT: Background and aims. Blastocystis is an intestinal protist with a broad host range and high
prevalence in human population worldwide. Next-generation sequencing (NGS) and
metataxonomic based studies of microorganisms of public health significance has grown
considerably in recent years, particularly for prokaryotes. However, a few studies have been
reported for eukaryotes like Blastocystis. The aim of this study was to evaluate the presence of
Blastocystis in fecal samples from Colombian children through a 18S ribosomal gene
metataxonomic approach. Methods. Twenty-eight stool samples from children <5 years from
daycare centers from Medellin (Colombia), and with a microscopic diagnosis for Blastocystis were
included in the study. DNA was extracted from fecal samples and the V3-V4 hypervariable regions
of the 18S rRNA gene was amplified. Sequences were obtained on the Illumina MiSeq platform.
Depurated reads were grouped into OTUs and the relative abundance and taxonomic rank for
eukaryotes were calculated. Results. Metataxonomic experiment detected Blastocystis in the 28
samples evaluated. Additionally, a taxonomic coverage for other intestinal parasites was achieved,
including Cryptosporidium, Giardia, Dientamoeba fragilis, and ascaridida nematodes. Conclusions.
NGS-based approach enabled intestinal parasites detection and has the potential to evaluate the
genetic diversity of eukaryotes in clinical samples. | |
| dc.description | COL0058784 | |
| dc.description | COL0040189 | |
| dc.format | 1 | |
| dc.format | application/pdf | |
| dc.format | application/pdf | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Grupo de Investigación Clínica en Enfermedades del Niño y del Adolescente - Pediaciencias | |
| dc.publisher | Microbiología Ambiental | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | Atribución-NoComercial-CompartirIgual 2.5 Colombia | |
| dc.rights | http://creativecommons.org/licenses/by-nc-sa/2.5/co/ | |
| dc.rights | http://purl.org/coar/access_right/c_abf2 | |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/4.0/ | |
| dc.subject | Blastocystis | |
| dc.subject | Secuenciación de Nucleótidos de Alto Rendimiento | |
| dc.subject | High-Throughput Nucleotide Sequencing | |
| dc.subject | Gusanos parásitos e intestinales | |
| dc.subject | Worms, intestinal and parasitic | |
| dc.subject | Parásitos intestinales | |
| dc.subject | Intestinal Parasites | |
| dc.subject | Metataxonómico | |
| dc.subject | Metataxonómic | |
| dc.title | Eukaryotic Metataxonomic Analysis of Faeces for the Identification of Blastocystis and Other Intestinal Parasites | |
| dc.type | info:eu-repo/semantics/conferenceObject | |
| dc.type | info:eu-repo/semantics/publishedVersion | |
| dc.type | http://purl.org/coar/resource_type/c_5794 | |
| dc.type | https://purl.org/redcol/resource_type/EC | |
| dc.type | Documento de conferencia | |
