Discrimination of Seven Anopheles Species from San Pedro de Urabá, Antioquia, Colombia, by Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Analysis of Its Sequences

dc.creatorZapata Tamayo, Mario Augusto
dc.creatorCienfuegos Gallet, Astrid Vanessa
dc.creatorQuirós, Óscar Iván
dc.creatorQuiñones Pinzón, Martha Lucía
dc.creatorLuckhart, Shirley
dc.creatorCorrea Ochoa, Margarita María
dc.date2021-08-01T15:42:33Z
dc.date2021-08-01T15:42:33Z
dc.date2007
dc.date.accessioned2023-08-28T19:45:42Z
dc.date.available2023-08-28T19:45:42Z
dc.identifierZapata MA, Cienfuegos AV, Quirós OI, Quiñones ML, Luckhart S, Correa MM. Discrimination of seven Anopheles species from San Pedro de Uraba, Antioquia, Colombia, by polymerase chain reaction-restriction fragment length polymorphism analysis of its sequences. Am J Trop Med Hyg. 2007 Jul;77(1):67-72.
dc.identifier0002-9637
dc.identifierhttp://hdl.handle.net/10495/21379
dc.identifier1476-1645
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8468000
dc.descriptionABSTRACT: Accurate identification of anopheline species is essential for vector incrimination and implementation of appropriate control strategies. Several anopheline species are considered important malaria vectors in Colombia; however, species determination is complicated by cryptic morphology and intra-individual variation. We describe polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) of internal transcribed spacer 2 (ITS2) sequences for differentiation of seven Anopheles species collected in a locality in Antioquia, Colombia, with high levels of malaria transmission. Each of these seven species can be identified by unique AluI PCR-RFLP restriction patterns. Comparisons of morphologic identification with molecular identification of voucher specimens confirmed species designation for 886 wild-caught anophelines. This new method can be used as a diagnostic tool for discrimination of anopheline species of medical importance in this region, some of which have overlapping morphologic characters and for conducting complementary studies where rapid and accurate identification of large numbers of specimens is needed.
dc.descriptionCOL0015099
dc.descriptionCOL0013746
dc.format6
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherAmerican Society of Tropical Medicine and Hygiene
dc.publisherMicrobiología Molecular
dc.publisherPrograma de Estudio y Control de Enfermedades Tropicales (PECET)
dc.publisherBaltimore, Estados Unidos
dc.relationAm. J. Trop. Med. Hyg.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by/4.0/
dc.subjectMalaria
dc.subjectAnopheles
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectPolymerase Chain Reaction
dc.titleDiscrimination of Seven Anopheles Species from San Pedro de Urabá, Antioquia, Colombia, by Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Analysis of Its Sequences
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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