Comparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells

dc.creatorPérez Cardona, Juan Manuel
dc.creatorMaldonado Celis, María Elena
dc.creatorRojano, Benjamín Alberto
dc.creatorAlzate Guarín, Fernando
dc.creatorSáez Vega, Jairo Antonio
dc.creatorCardona Galeano, Wilson
dc.date2019-09-27T18:45:58Z
dc.date2019-09-27T18:45:58Z
dc.date2014
dc.date.accessioned2023-08-28T19:34:08Z
dc.date.available2023-08-28T19:34:08Z
dc.identifierPérez-Cardona J, Maldonado-Celis M, Rojano B, Alzate-Guarín F, Sáez-Vega J, Cardona-Galeano W. Comparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells. Trop J Pharm Res. 2014; 13(8): 1279-86. DOI: 10.4314/tjpr.v13i8.12
dc.identifier1596-5996
dc.identifierhttp://hdl.handle.net/10495/12041
dc.identifier10.4314/tjpr.v13i8.12
dc.identifier1596-9827
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8463893
dc.descriptionABSTRACT: To determine and compare the antioxidant, antiproliferative and apoptotic effects of leaf infusions of Ilex laurina and Ilex paraguariensis in colon cancer cells. Methods: Antioxidant activity was determined by ORAC (Oxygen Radical Absorbance Capacity) and FRAP (Ferric Reducing Antioxidant Power). Cytotoxic and antiproliferative effects were analyzed using MTT ((3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) and sulfhorodamine-B respectively. Cell death and apoptosis of human colon adenocarcinoma cells SW480 and their metastatic-derived SW620 cells, were analyzed by flow cytometry using propidium iodide and Annexin-V. Results: Although their flavonoid levels were similar, I. laurina infusion contained 2.2 and 4.4 times higher amounts of total phenolic and caffeoyl derivatives, respectively, than I. paraguariensis. FRAP and ORAC values for I. laurina infusion were 1.6 and 2.0 more active than I. paraguariensis. Both plant infusions inhibited viability and cell growth of SW480 and SW620 cells. These results may be associated to cell cycle-arrest and apoptosis because of the comparable increase of hypodiploid and annexin-V positive colon cancer cells. Conclusion: These data highlight the antioxidant and promising anticancer activities of I. laurina and Ilex paraguariensis.
dc.descriptionCOL0008227
dc.descriptionCOL0083811
dc.descriptionCOL0015329
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherUniversity of Benin, Faculty of Pharmacy
dc.publisherGrupo de Estudios Botánicos
dc.publisherImpacto de Componentes Alimentarios en la Salud
dc.publisherQuímica de Plantas Colombianas
dc.publisherCiudad de Benín, Nigeria
dc.relationTrop. J. Pharm. Res.
dc.rightsAtribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectIlex laurina
dc.subjectIlex paraguariensis
dc.subjectAntioxidant
dc.subjectAntiproliferative
dc.subjectApoptosis
dc.subjectColon cancer
dc.titleComparative antioxidant, antiproliferative and apoptotic effects of Ilex laurina and Ilex paraguariensis on colon cancer cells
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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