Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection

dc.creatorCastillo Ramírez, Jorge Andrés
dc.creatorGiraldo Giraldo, Diana Marcela
dc.creatorRodenhuis Zybert, Izabela Agata
dc.creatorSmit, Jolanda
dc.creatorUrcuqui Inchima, Silvio
dc.date2022-07-14T15:44:10Z
dc.date2022-07-14T15:44:10Z
dc.date2022
dc.date.accessioned2023-08-28T19:33:06Z
dc.date.available2023-08-28T19:33:06Z
dc.identifierCastillo JA, Giraldo DM, Smit JM, Rodenhuis-Zybert IA, Urcuqui-Inchima S. Vitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection. Pathog Dis. 2022 May 23;80(1):ftac014. doi: 10.1093/femspd/ftac014.
dc.identifierhttps://hdl.handle.net/10495/29734
dc.identifier10.1093/femspd/ftac014
dc.identifier2049-632X
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8463486
dc.descriptionABSTRACT: Epidemics of dengue, an acute and potentially severe disease caused by mosquito-borne dengue virus (DENV), pose a major challenge to clinicians and health care services across the sub(tropics). Severe disease onset is associated with a dysregulated inflammatory response to the virus, and there are currently no drugs to alleviate disease symptoms. LL-37 is a potent antimicrobial peptide with a wide range of immunoregulatory properties. In this study, we assessed the effect of LL-37 on DENV-2-induced responses in human monocyte-derived macrophages (MDMs). We show that simultaneous exposure of exogenous LL-37 and DENV-2 resulted in reduced replication of the virus in MDMs, while the addition of LL-37 postexposure to DENV-2 did not. Interestingly, the latter condition reduced the production of IL-6 and increased the expression of genes involved in virus sensing and antiviral response. Finally, we demonstrate that low endogenous levels and limited production of LL-37 in MDMs in response to DENV-2 infection can be increased by differentiating MDMs in the presence of Vitamin D (VitD3). Taken together, this study demonstrates that in addition to its antimicrobial properties, LL-37 has immunomodulatory properties in the curse of DENV infection and its production can be increased by VitD3.
dc.descriptionCOL0012444
dc.format11
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherOxford University Press
dc.publisherInmunovirología
dc.publisherOxford, Estados Unidos
dc.relationPathog. Dis.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAtribución-NoComercial-CompartirIgual 2.5 Colombia
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/2.5/co/
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectVirus del Dengue
dc.subjectDengue Virus
dc.subjectMacrófagos
dc.subjectMacrophages
dc.subjectInmunidad Innata
dc.subjectImmunity, Innate
dc.subjectVitamina D
dc.subjectVitamin D
dc.subjectLL-37
dc.titleVitamin D-induced LL-37 modulates innate immune responses of human primary macrophages during DENV-2 infection
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/acceptedVersion
dc.typehttp://purl.org/coar/resource_type/c_2df8fbb1
dc.typehttps://purl.org/redcol/resource_type/ART
dc.typeArtículo de investigación


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