| dc.creator | Rodr?guez Almonacid, Cristian Camilo | |
| dc.date | 2019-04-24T23:56:24Z | |
| dc.date | 2019-04-24T23:56:24Z | |
| dc.date | 2017 | |
| dc.date.accessioned | 2023-08-28T13:33:34Z | |
| dc.date.available | 2023-08-28T13:33:34Z | |
| dc.identifier | Rodr?guez Almonacid, Cristian Camilo. Estudio comparativo del perfil prote?mico de epimastigotes largos y cortos de Trypanosoma rangeli KP1(+) Y KP1(-). Ibagu? : Universidad del Tolima, 2017 | |
| dc.identifier | https://repository.ut.edu.co/handle/001/2669 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8440133 | |
| dc.description | 133 p. Recurso Electr?nico | |
| dc.description | Trypanosoma rangeli es un par?sito protozoario que hasta ahora es considerado
inocuo para el hospedero vertebrado pero perjudicial para el vector. Estudios han
revelado que sus subpoblaciones son transmitidas selectivamente por las
especies del g?nero Rhodnius, asimismo, se han descrito diferencias en la
sensibilidad de los epimastigotes cortos y largos a la lisis mediada por la
hemolinfa de R. prolixus. Debido a esto, se considera que existen factores
proteicos en el par?sito que podr?an estar involucrados en dicha susceptibilidad
o resistencia. Por esta raz?n, el objetivo del presente trabajo fue analizar los
perfiles electrofor?ticos unidimensionales y bidimensionales de las prote?nas
expresadas por los epimastigotes cortos y largos de las subpoblaciones KP1(+)
y KP1(-) de T. rangeli.
Se usaron las cepas Choach? (KP1+/A) y Rcol 0201 (KP1-/C); estas se
masificaron durante 7 y 14 d?as para obtener epimastigotes cortos y largos
respectivamente. Posteriormente fueron lisados y la prote?na fue cuantificada por
el m?todo Bradford. La electroforesis por SDS-PAGE se realiz? en geles al 12%,
mientras que la separaci?n bidimensional se realiz? por isoelectroenfoque y una
posterior SDS-PAGE. Los geles unidimensionales fueron analizados con los
programas Image Lab?, RAPDplot y PHYLIP96 y los geles bidimensionales con
PDQuest? Advanced.
Los perfiles unidimensionales mostraron que la mayor?a de prote?nas poseen un
peso molecular entre 20-70 kDa. Los dendrogramas basados en an?lisis de
identidad exhibieron diferencias entre las subpoblaciones y entre epimastigotes
cortos y largos. Los perfiles bidimensionales mostraron spots exclusivos para
cada una de las muestras que podr?an estar involucrados con la interacci?n
par?sito-vector.
Palabras clave: T. rangeli, SDS-PAGE, electroforesis bidimensional, prote?na,
epimastigotes | |
| dc.description | Trypanosoma rangeli is a protozoan parasite that to date it is considered an
innocuous parasite for the vertebrate host but detrimental for the vector. Studies
have revealed that its subpopulations are selectively transmitted by species of the
genus Rhodnius, likewise, differences in the sensitivity of short and long
epimastigotes to lysis mediated by hemolymph of R. prolixus have been
described. Because of this, it is considered that there are protein factors in the
parasite that could be involved in such susceptibility or resistance. Because of
this, the aim of this work was to analyze the one - dimensional and two -
dimensional electrophoretic profiles of the proteins expressed by short and long
epimastigotes of the KP1 (+) and KP1 (-) subpopulations of T. rangeli.
Choach? (KP1 + / A) and Rcol 0201 (KP1- / C) strains were used; these were
massified for 7 and 14 days to obtain short and long epimastigotes respectively.
They were subsequently lysed and the protein was quantified by the Bradford
method. SDS-PAGE electrophoresis was performed on 12% gels, while the twodimensional
separation was performed by isoelectric focusing and a subsequent
SDS-PAGE. One-dimensional gels were analyzed with the Image Lab ?,
RAPDplot and PHYLIP96 software packages and the two-dimensional gels with
PDQuest ? Advanced software.
The one-dimensional profiles showed that most proteins have a molecular weight
between 20-70 kDa. Dendrograms based on identity analysis exhibited
differences between subpopulations and between short and long epimastigotes.
The two-dimensional profiles showed unique spots for each sample that might be
involved with the parasite-vector interaction.
Keywords: T. rangeli, SDS-PAGE, two-dimensional electrophoresis, proteins,
epimastigotes. | |
| dc.format | application/pdf | |
| dc.format | application/pdf | |
| dc.language | spa | |
| dc.publisher | Ibagu? : Universidad del Tolima, 2017 | |
| dc.publisher | (CO COL 170) | |
| dc.rights | Atribuci?n-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2-5 CO) | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights | http://www.creativecommons.org/licenses by-nc/2.5/co/ | |
| dc.subject | T. rangeli | |
| dc.subject | SDS-PAGE | |
| dc.subject | electroforesis bidimensiona | |
| dc.subject | prote?na | |
| dc.title | Estudio comparativo del perfil prote?mico de epimastigotes largos y cortos de Trypanosoma rangeli | |
| dc.type | Trabajo de grado - Pregrado | |
| dc.type | Text | |
| dc.type | info:eu-repo/semantics/bachelorThesis | |
| dc.type | info:eu-repo/semantics/submittedVersion | |
| dc.coverage | (Ibagu? - Tolima - Colombia) | |
| dc.coverage | 2019-04-24T16:00Z | |
| dc.coverage | (Mundo, Suram?rica, Colombia, Tolima) [1023837] | |
