INFLUENCE OF SODIUM GLUTAMATE, BUBBLING N2- GAS AND SUPERFICIAL AERATION ON TETANUS TOXIN PRODUCTION IN Clostridium tetani CULTURES

dc.contributornull
dc.creatorGutiérrez, I.; Laboratorio de Tétanos, Instituto Nacional de Salud.
dc.creatorGarzón, E.; Laboratorio de Tétanos, Instituto Nacional de Salud.
dc.creatorVargas, P.; Laboratorio de Tétanos, Instituto Nacional de Salud.
dc.creatorMoreno, N.; Grupo de Fermentaciones, Instituto de Biotecnología, Universidad Nacional de Colombia.
dc.creatorPoutou Piñales, Raúl Alberto; Laboratorio de Biotecnología Aplicada, Grupo de Biotecnología Ambiental e Industrial, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
dc.date2018-02-24T15:59:17Z
dc.date2020-04-15T18:10:07Z
dc.date2023-05-11T17:12:45Z
dc.date2018-02-24T15:59:17Z
dc.date2020-04-15T18:10:07Z
dc.date2023-05-11T17:12:45Z
dc.date2005-07-10
dc.date.accessioned2023-08-24T07:21:16Z
dc.date.available2023-08-24T07:21:16Z
dc.identifierhttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/4984
dc.identifier2027-1352
dc.identifier0122-7483
dc.identifierhttps://hdl.handle.net/20.500.12032/105334
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8411443
dc.descriptionThe influence of sodium glutamate as a supplement to Latham Mueller medium, while using bubbling nitrogen flow as an anaerobic agent and superficial aeration as an inducer of cell lysis and as a mechanism for the haulage of gases in the fermentation processes was evaluated. Using the Clostridium tetani Massachusetts’s strain, several five (5) liter batch fermentations were carried out for tetanus toxin production under the following conditions: Latham Mueller medium, with or without sodium glutamate, nitrogen flow and superficial aeration. The results demonstrated that the addition of sodium glutamate (2.5 g/l), combined with a bubbling nitrogen flow (0.33 l/min) and superficial aeration (0.33 l/min), produced asignificant increase in cell concentrations, repressing the tetanus toxin formation; while the gas flow (nitrogen and superficial aeration) without sodium glutamate improved the toxin production by approximately 49%, providing conditions for the following outcomes: a maximum toxin level of 73 Lf/ml; a toxin formation rate of 1844.0 Lf/l.h; and, an over-all productivity of 833.5 Lf/l.h.
dc.formatPDF
dc.formatapplication/pdf
dc.languageeng
dc.publisherPontificia Universidad Javeriana
dc.relationhttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/4984/3840
dc.subjectnull
dc.subjecttetanus toxin; fermentation; N2; sodium glutamate
dc.subjectnull
dc.titleINFLUENCE OF SODIUM GLUTAMATE, BUBBLING N2- GAS AND SUPERFICIAL AERATION ON TETANUS TOXIN PRODUCTION IN Clostridium tetani CULTURES


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