Videos
Tomographic reconstruction [Secretory pathway; 7b]
Autor
Ladinsky, Mark S.
Howell, Kathryn E.
Resumen
Presents tomographic reconstruction of a portion of the Golgi complex in a Normal Rat Kidney (NRK) cell. The tomogram was calculated from a series of tilted views of a 1 micron section, imaged on a 1 MeV high voltage electron microscope. The Golgi stack is comprised of seven cisternae, but only the seventh (trans-most) cisterna displays clathrin coated budding profiles. Clathrin spikes are clearly seen on equatorial views of certain buds. When these buds are followed to their edges through the volume of the tomogram, the distinctive 'cage' of the clathrin triskelions comes into view Componente Curricular::Educação Superior::Ciências Biológicas::Biologia Geral