A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives.

dc.creatorParkhouse, Michael
dc.creatorCampoverde Cisneros, Manuel Alfredo
dc.creatorHarrison, Leslie
dc.creatorCarpio Rodas, Luis Arturo
dc.creatorCortéz, María Milagros
dc.creatorRojas, Glenda C.
dc.creatorSastre, Patricia
dc.date.accessioned2023-07-12T17:25:46Z
dc.date.accessioned2023-08-10T14:47:47Z
dc.date.available2023-07-12T17:25:46Z
dc.date.available2023-08-10T14:47:47Z
dc.date.created2023-07-12T17:25:46Z
dc.date.issued2019
dc.identifier0035-9203
dc.identifierhttp://dspace.ucuenca.edu.ec/handle/123456789/42383
dc.identifierhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061121773&origin=inward
dc.identifier10.1093/trstmh/try116.
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8152094
dc.description.abstractBackground Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was …
dc.languagees_ES
dc.sourceTransactions of the Royal Society of Tropical Medicine and Hygiene
dc.subjectNeurocysticercosis (NCC)
dc.subjectField diagnosis
dc.subjectBovine cysticercosis
dc.subjectHP10 antigen ELISA
dc.subjectHP10 antigen LFA
dc.titleA modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives.
dc.typeARTÍCULO


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