Prevalence and detection by nested PCR of Anaplasma marginale in cattle and tick in the center of the coast of Ecuador
Prevalencia y detección por PCR anidada de Anaplasma marginale en bovinos y garrapatas en la zona central del Litoral ecuatoriano
| dc.creator | Escobar Troya, Ariel | |
| dc.creator | Pinargote Mendoza, Edgar | |
| dc.creator | Carranza Patiño, Helen | |
| dc.creator | Carranza Patiño, Mercedes Susana | |
| dc.creator | Cevallos Falquez, Orly Fernando | |
| dc.creator | Villarreal, Patricio | |
| dc.date | 2015-09-15 | |
| dc.date.accessioned | 2023-08-09T21:37:04Z | |
| dc.date.available | 2023-08-09T21:37:04Z | |
| dc.identifier | https://revistas.uteq.edu.ec/index.php/cyt/article/view/145 | |
| dc.identifier | 10.18779/cyt.v8i1.145 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8135240 | |
| dc.description | The bacterium causing anaplasmosis in cattle is known as Anaplasma marginale. It is mainly transmitted biologically by ticks, flies, and fomites; it can also be transmitted through infected blood as a result of improper use of surgical tools. Up to this moment, Ecuador lacks updated studies on efficient procedures for specific A. marginale diagnosis and eradication through the control of these insects, therefore it is necessary to develop and implement proposals related to the use of this molecular tool for potential vectors of transmission of this disease in cattle. For this work, DNA was extracted efficiently by the method of Salting Out. A total of 255 samples were analyzed by Nested PCR, distributed as follows |108| Rhipicephalus (Boophilus) microplus, |85| bovine, from these, the 13.46% and 85.48% were positive for rickettsia, the samples of Amblyomma spp |62| all were negative. The total Kappa index of infested cattle against Rhipicephalus (Boophilus) microplus|, was not significant (0.28). Subsequently,the presence or absence of disease which was determined by χ2 (p = 0.66) does not depend on the location from where the cattle comes | en-US |
| dc.description | La bacteria que provoca la anaplasmosis en bovinos se conoce como Anaplasma marginale, es potencialmente transmitida de forma biológica por garrapatas, moscas y fómites o mediante sangre infectada como consecuencia del uso incorrecto de herramientas quirúrgicas. Hasta la fecha, Ecuador carece de estudios actualizados sobre procedimientos eficientes para el diagnóstico específico y, erradicación de A. marginale a través del control de estos insectos, por lo que resulta de gran importancia desarrollar e implementar trabajos relacionados con el uso de esta herramienta molecular, para la detección de la bacteria en vectores de transmisión que provocan la enfermedad en bovinos. En este trabajo, se extrajo ADN eficazmente con el método de Salting Out. Un total de 255 muestras fueron analizadas por PCR anidada, distribuidas del siguiente modo|108| Riphicephalus (Boophilus) microplus, |85| bovinos, de estos el 13.46% y 85.48% dieron positivas para la rickettsia, las muestras de Amblyomma spp. |62| todas fueron negativas. El índice de concordancia Kappa |total de bovinos infestados frente a Riphicephalus (Boophilus) microplus|, no fue significativo (0.28). Subsiguientemente, se determinó por χ2 (p=0.66) que la presencia o ausencia de la enfermedad es independiente del lugar de donde proviene el bovino. | es-ES |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Universidad Técnica Estatal de Quevedo | es-ES |
| dc.relation | https://revistas.uteq.edu.ec/index.php/cyt/article/view/145/159 | |
| dc.rights | https://creativecommons.org/licenses/by-nc-sa/4.0 | es-ES |
| dc.source | Science and Technology; Vol. 8 No. 1 (2015): January-June (2015); 11-17 | en-US |
| dc.source | Ciencia y Tecnología; Vol. 8 Núm. 1 (2015): Enero-Junio (2015); 11-17 | es-ES |
| dc.source | Ciência e Tecnologia; v. 8 n. 1 (2015): Enero-Junio (2015); 11-17 | pt-BR |
| dc.source | 1390-4043 | |
| dc.source | 1390-4051 | |
| dc.source | 10.18779/cyt.v8i1 | |
| dc.subject | DETECCIÓN | es-ES |
| dc.subject | BOVINOS | es-ES |
| dc.subject | FINCAS | es-ES |
| dc.subject | SALTING OUT. | es-ES |
| dc.subject | DETECTION | en-US |
| dc.subject | CATTLE | en-US |
| dc.subject | FARMS | en-US |
| dc.subject | SALTING OUT. | en-US |
| dc.title | Prevalence and detection by nested PCR of Anaplasma marginale in cattle and tick in the center of the coast of Ecuador | en-US |
| dc.title | Prevalencia y detección por PCR anidada de Anaplasma marginale en bovinos y garrapatas en la zona central del Litoral ecuatoriano | es-ES |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | info:eu-repo/semantics/publishedVersion |