Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay

dc.creatorMaschmann, Raquel de Abreu
dc.creatorVerza, Mirela
dc.creatorSilva, Marcia Susana Nunes
dc.creatorSperhacke, Rosa Dea
dc.creatorRibeiro, Marta Osório
dc.creatorSuffys, Philip Noel
dc.creatorGomes, Harrison Magdinier
dc.creatorTortoli, Enrico
dc.creatorMarcelli, Fiorella
dc.creatorZaha, Arnaldo
dc.creatorRossetti, Maria Lucia Rosa
dc.date2011-08-04T06:01:20Z
dc.date2011
dc.identifier0074-0276
dc.identifierhttp://hdl.handle.net/10183/30563
dc.identifier000776671
dc.descriptionWe used a colorimetric reverse dot blot hybridization (CRDH) assay to detect the presence of mutations in a specific region of the rpoB gene, associated with rifampin (RIF) resistance, in a panel of 156 DNAs extracted from 103 RIF-sensitive and 53 RIF-resistant cultures of Mycobacterium tuberculosis. When compared with the antimicrobial susceptibility test (AST), the sensitivity and specificity of the CRDH were 92.3% and 98.1%, respectively. When compared with sequencing, the sensitivity and specificity of the CRDH were 90.6% and 100%, respectively. To evaluate the performance of the assay directly in clinical specimens, 30 samples from tuberculosis patients were used. For these samples, the results of the CRDH were 100% consistent with the results of the AST and sequencing. These results indicate that the rate of concordance of the CRDH is high when compared to conventional methods and sequencing data. The CRDH can be successfully applied when a rapid test is required for the identification of RIF resistance in M. tuberculosis.
dc.formatapplication/pdf
dc.languageeng
dc.relationMemorias do Instituto Oswaldo Cruz : an International Journal of Biological and Biomedical Research. Rio de Janeiro. Vol. 106, n. 2 (mar. 2011), p. 139-145
dc.rightsOpen Access
dc.subjectDrug resistance
dc.subjectRenverse dot blot hybridization
dc.subjectRifampin
dc.subjectrpoB
dc.subjectTuberculosis
dc.subjectMycobacterium tuberculosis
dc.subjectTuberculose
dc.subjectHibridização
dc.subjectRifampicina
dc.titleDetection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
dc.typeArtigo de periódico
dc.typeNacional


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