Redundancy and recombination in the Echinococcus AgB multigene family : is there any similarity with protozoan contigency genes?

dc.creatorHaag, Karen Luisa
dc.creatorGottstein, Bruno
dc.creatorMuller, Norbert
dc.creatorSchnorr, André
dc.creatorAyala, Francisco J.
dc.date2011-01-21T05:59:02Z
dc.date2006
dc.identifier0031-1820
dc.identifierhttp://hdl.handle.net/10183/27460
dc.identifier000588215
dc.descriptionNumerous genetic variants of the Echinococcus antigen B (AgB) are encountered within a single metacestode. This could be a reflection of gene redundancy or the result of a somatic hypermutation process. We evaluate the complexity of the AgB multigene family by characterizing the upstream promoter regions of the 4 already known genes (EgAgB1-EgAgB4) and evaluating their redundancy in the genome of 3 Echinococcus species (E. granulosus, E. ortleppi and E. multilocularis) using PCR-based approaches. We have ascertained that the number of AgB gene copies is quite variable, both within and between species. The most repetitive gene seems to be AgB3, of which there are more than 110 copies in E. ortleppi. For E. granulosus, we have cloned and characterized 10 distinct upstream promoter regions of AgB3 from a single metacestode. Our sequences suggest that AgB1 and AgB3 are involved in gene conversion. These results are discussed in light of the role of gene redundancy and recombination in parasite evasion mechanisms of host immunity, which at present are known for protozoan organisms, but virtually unknown for multicellular parasites.
dc.formatapplication/pdf
dc.languageeng
dc.relationParasitology. Cambridge, Inglaterra. Vol. 133, n. 4 (Oct. 2006), p. 411-419
dc.rightsOpen Access
dc.subjectHydatid disease
dc.subjectAntigen B
dc.subjectPromoter
dc.subjectRecombination
dc.subjectAntigen diversity
dc.subjectReal-time PCR
dc.subjectEchinococcus
dc.subjectHidatidose : Antigenos : Imunodiagnostico
dc.subjectAntígeno B
dc.titleRedundancy and recombination in the Echinococcus AgB multigene family : is there any similarity with protozoan contigency genes?
dc.typeArtigo de periódico
dc.typeEstrangeiro


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