The effect of sterilization using ethanol, ultraviolet radiation (UVR) and antibiotic solution in PLGA scaffolds produced by electrospinning was evaluated. The properties of nanofibers and on the cellular adhesion were analysed. All methods generated sterile scaffolds but led to some kind of damage. Ethanol and antibiotics caused changes in morphology and dimensions of scaffolds, which was not observed when using UVR. On the other hand, UVR caused a greater reduction of molecular weight which increased with exposure time. Nanofibres sterilized with antibiotics showed greater cellular adhesion than the others methods, demonstrating their potentiality as a method to sterilize PLGA nanofibers.