Reduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats

dc.creatorSouza, M. S.
dc.creatorMachado, Ubiratan Fabres
dc.creatorOkamoto, Maristela Mitiko
dc.creatorBertoluci, Marcello Casaccia
dc.creatorPonpermeyer, C.
dc.creatorLeguisamo, Natalia Motta
dc.creatorAzambuja, Felipe
dc.creatorIrigoyen, Maria Claudia Costa
dc.creatorSchaan, Beatriz D'Agord
dc.date2010-04-24T04:15:52Z
dc.date2008
dc.identifier0100-879X
dc.identifierhttp://hdl.handle.net/10183/21227
dc.identifier000673195
dc.descriptionDiabetes in spontaneously hypertensive rats is associated with cortical renal GLUT1 and GLUT2 overexpression. Our objective was to evaluate the effect of the angiotensin-converting enzyme blockade on cortical renal GLUT1 and GLUT2 expression, urinary albumin and urinary TGF-β1. Streptozotocin, 50 mg/kg, or citrate buffer (N = 16) was administered as a single injection into the tail vein in adult spontaneously hypertensive rats (~260 g). Thirty days later, these diabetic spontaneously hypertensive rats received ramipril by gavage: 0.01 mg·kg-1·day-1 (D0.01, N = 14), 1 mg·kg-1·day-1 (D1, N = 9) or water (D, N = 11) for 15 days. Albumin and TGF-β1 (24-h urine), direct arterial pressure, renal tissue angiotensin-converting enzyme activity (fluorometric assay), and GLUT1 and GLUT2 protein levels (Western blot, renal cortex) were determined. Glycemia and glycosuria were higher (P < 0.05) in the diabetic rats compared with controls, but similar between the diabetic groups. Diabetes in spontaneously hypertensive rats lowered renal tissue angiotensin-converting enzyme activity (40%), which was reduced further when higher ramipril doses were used. Diabetes associated with hypertension raised GLUT1 by 28% (P < 0.0001) and GLUT2 by 76% (P = 0.01), and both doses of ramipril equally reduced cortical GLUT1 (D vs D1 and vs D0.01, P ≤ 0.001). GLUT2 levels were reduced in D0.01 (P < 0.05 vs D). Diabetes increased urinary albumin and TGF-β1 urinary excretion, but the 15-day ramipril treatment (with either dose) did not reduce them. In conclusion, ramipril is effective in lowering renal tissue angiotensin-converting enzyme activity, as well as blocking cortical GLUT1 overexpression, which may be beneficial in arresting the development of diabetic nephropathy.
dc.formatapplication/pdf
dc.languageeng
dc.relationBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. RiBeirão Preto, SP. Vol. 41, n. 11 (Nov. 2008), p. 960-968
dc.rightsOpen Access
dc.subjectAngiotensin-converting enzyme inhibition
dc.subjectDiabetic nephropathy
dc.subjectGlucose transporter proteins
dc.subjectHypertension
dc.subjectStreptozotocin
dc.subjectInibidores da enzima conversora da angiotensina
dc.subjectDiabetes mellitus
dc.subjectHipertensão
dc.titleReduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats
dc.typeArtigo de periódico
dc.typeNacional


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