Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line

dc.creatorGimba, Etel Rodrigues Pereira
dc.creatorChemale, Gustavo
dc.creatorFarias, Sandra Estrazulas
dc.creatorZaha, Arnaldo
dc.date2010-04-24T04:15:32Z
dc.date2000
dc.identifier0100-879X
dc.identifierhttp://hdl.handle.net/10183/21159
dc.identifier000296773
dc.descriptionWe report here for the first time the structure and function of a promoter from a cestode. The ability of DNA fragments respectively encompassing the 935-bp and 524-bp regions upstream from the ATG codon from the EgactI and EgactII actin genes of Echinococcus granulosus to promote transcription was studied in the NIH3T3 mouse cell line. The results of transfection assays showed that both regions have strong promoter activity in these cells. The fragments were tested in both orientations and the 524-bp fragment of EgactII presented a bidirectional promoter activity. Deletion analysis of EgactI and EgactII promoters indicated the presence of regulatory regions containing putative silencer elements. These results indicate that both EgactI and EgactII promoters are functional and that the preliminary functional evaluation of E. granulosus and possibly of other cestode promoters can be performed in heterologous cell lines.
dc.formatapplication/pdf
dc.languageeng
dc.relationBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 33, no. 11 (Nov. 2000), p. 1283-1292
dc.rightsOpen Access
dc.subjectEchinococcus granulosus
dc.subjectActin
dc.subjectPromoters
dc.subjectCestode
dc.subjectGene expression
dc.subjectDeletions
dc.subjectGenética
dc.titleCloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line
dc.typeArtigo de periódico
dc.typeNacional


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