Peritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neolycoprotein

dc.creatorPassos, Eduardo Pandolfi
dc.creatorBrugnara, Laura
dc.creatorFacin, Andrea Cintra
dc.creatorRiffel, Alessandro
dc.creatorLima, Geraldo Rodrigues de
dc.creatorFreitas, V.
dc.creatorBrandelli, Adriano
dc.date2010-04-24T04:15:31Z
dc.date1999
dc.identifier0100-879X
dc.identifierhttp://hdl.handle.net/10183/21153
dc.identifier000294542
dc.descriptionThe effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophoretreated sperm showed that NGP-induced exocytosis differed significantly between the PFc (11%) and PFe/PFi groups (17%), and the ionophore-induced AR was higher for PFi (33%) than PFc/PFe (25%). The incidence of the NGP-induced AR was reduced in the first hour of pre-incubation with PFc and remained nearly constant throughout 4 h of incubation. The present data indicate that PF possesses a protective factor which prevents premature AR.
dc.formatapplication/pdf
dc.languagepor
dc.relationBrazilian journal of medical and biological research. Ribeirão Preto, SP. Vol. 32, no. 1 (Jan. 1999), p. 59-65
dc.rightsOpen Access
dc.subjectEndometriose
dc.subjectN-acetilglucosaminidase
dc.subjectReação acrossômica
dc.subjectInfertilidade
dc.subjectFluido peritoneal
dc.subjectExocitose
dc.subjectAcrosome reaction
dc.subjectEndometriosis
dc.subjectInfertility
dc.subjectPeritoneal fluid
dc.subjectSperm
dc.titlePeritoneal fluid modulates the sperm acrosomal exocytosis induced by N-acetylglucosaminyl neolycoprotein
dc.typeArtigo de periódico
dc.typeNacional


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