Intercellular contact-dependent survival of human A549, NCI-H596 and NCI-H520 non-small cell lung carcinoma cell lines

dc.creatorCastro, Mauro Antônio Alves
dc.creatorSchwartsmann, Gilberto
dc.creatorMoreira, Jose Claudio Fonseca
dc.date2010-04-24T04:15:28Z
dc.date2001
dc.identifier0100-879X
dc.identifierhttp://hdl.handle.net/10183/21138
dc.identifier000327080
dc.descriptionIn the present study, we examined the relationship between cell phenotype and cell survival of three human non-small cell lung carcinoma cell lines (A549, NCI-H596 and NCI-H520). Cells in exponential growth at various densities were incubated for 24 h at 37ºC in a 5% CO2 humidified atmosphere and then exposed to UV radiation for 1 min (256 nm, 40 W, source-to-target distance 100 cm). After two days the surviving cells were quantified by sulforhodamine ß staining and DNA fragmentation assay. The differences in UV sensitivity at 60 x 103 cells/cm2 among the cell lines were not related to the proliferative state of the cells but to the extent of intercellular contact. In contrast to A549 and NCI-H596, irradiated NCI-H520 cells presented lower DNA fragmentation and an aggregated cell culture phenotype even prior to confluence, suggesting that a contact-effect mechanism provides further protection against UV radiation.
dc.formatapplication/pdf
dc.languageeng
dc.relationBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 34, no. 8 (Aug. 2001), p. 1007-1013
dc.rightsOpen Access
dc.subjectNon-small cell lung carcinoma
dc.subjectCell density
dc.subjectIntercellular contact
dc.subjectCell phenotype
dc.subjectUV radiation
dc.subjectNeoplasias pulmonares
dc.subjectCarcinoma pulmonar de células não pequenas
dc.subjectContagem de células
dc.subjectCiclo celular
dc.subjectFenótipo
dc.subjectTolerância à radiação
dc.titleIntercellular contact-dependent survival of human A549, NCI-H596 and NCI-H520 non-small cell lung carcinoma cell lines
dc.typeArtigo de periódico
dc.typeNacional


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