dc.creator | Castro, Mauro Antônio Alves | |
dc.creator | Schwartsmann, Gilberto | |
dc.creator | Moreira, Jose Claudio Fonseca | |
dc.date | 2010-04-24T04:15:28Z | |
dc.date | 2001 | |
dc.identifier | 0100-879X | |
dc.identifier | http://hdl.handle.net/10183/21138 | |
dc.identifier | 000327080 | |
dc.description | In the present study, we examined the relationship between cell phenotype and cell survival of three human non-small cell lung carcinoma cell lines (A549, NCI-H596 and NCI-H520). Cells in exponential growth at various densities were incubated for 24 h at 37ºC in a 5% CO2 humidified atmosphere and then exposed to UV radiation for 1 min (256 nm, 40 W, source-to-target distance 100 cm). After two days the surviving cells were quantified by sulforhodamine ß staining and DNA fragmentation assay. The differences in UV sensitivity at 60 x 103 cells/cm2 among the cell lines were not related to the proliferative state of the cells but to the extent of intercellular contact. In contrast to A549 and NCI-H596, irradiated NCI-H520 cells presented lower DNA fragmentation and an aggregated cell culture phenotype even prior to confluence, suggesting that a contact-effect mechanism provides further protection against UV radiation. | |
dc.format | application/pdf | |
dc.language | eng | |
dc.relation | Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 34, no. 8 (Aug. 2001), p. 1007-1013 | |
dc.rights | Open Access | |
dc.subject | Non-small cell lung carcinoma | |
dc.subject | Cell density | |
dc.subject | Intercellular contact | |
dc.subject | Cell phenotype | |
dc.subject | UV radiation | |
dc.subject | Neoplasias pulmonares | |
dc.subject | Carcinoma pulmonar de células não pequenas | |
dc.subject | Contagem de células | |
dc.subject | Ciclo celular | |
dc.subject | Fenótipo | |
dc.subject | Tolerância à radiação | |
dc.title | Intercellular contact-dependent survival of human A549, NCI-H596 and NCI-H520 non-small cell lung carcinoma cell lines | |
dc.type | Artigo de periódico | |
dc.type | Nacional | |