Characterization of differently expressed genes during the interaction between ‘Fuji’ apples and Botryosphaeria dothidea

dc.creatorCorrent, Adriana Regina
dc.creatorSchwarz, Lígia Loss
dc.creatorOster, Andréia Hansen
dc.creatorMoraes, Marcelo Gravina de
dc.creatorBender, Renar João
dc.date2010-04-16T09:16:34Z
dc.date2009
dc.identifier0100-2945
dc.identifierhttp://hdl.handle.net/10183/20777
dc.identifier000698121
dc.descriptionO objetivo deste estudo foi investigar a interação entre o fungo Botryosphaeria dothidea e maçãs cv. Fuji por meio da técnica de Differential Display RT-PCR. O cDNA de frutos infectados e não infectados pelo fungo foi amplificado com uma combinação de 15 oligonucleotídeos iniciadores. Foram isolados 400 fragmentos de cDNA diferencialmente expressos, dos quais 120 foram sequenciados e comparados com sequências disponíveis no GenBank, por meio do programa BLASTX. As sequências obtidas foram similares à metalotioninas, profilina alergênica, proteína de resistência e fosfatase.
dc.descriptionThe aim of this study was to investigate the interaction between the Botryosphaeria dothidea fungi and ‘Fuji’ apples using the Differential Display RT-PCR technique. The cDNA of infected and non infected fruits by the fungus was amplified using a combination of 15 primers. Four hundred fragments of differentially expressed cDNA were isolated, and 120 of them were sequenced and compared with available sequences at GenBank database, using BLASTX software. Obtained sequences were similar to methalothionines, an allergenic profiline, a resistance linked protein, and a phosphatase.
dc.formatapplication/pdf
dc.languagepor
dc.relationRevista Brasileira de Fruticultura. Cruz das Almas, Ba. Vol. 31, n. 1 (mar. 2009), p. 268-272
dc.rightsOpen Access
dc.subjectMaçã
dc.subjectPodridão branca
dc.subjectDoença de planta
dc.subjectMalus domestica
dc.subjectWhite-rot
dc.subjectReverse transcriptase
dc.titleCaracterização de genes diferencialmente expressos na interação entre maçã 'Fuji' e Botryosphaeria dothidea
dc.titleCharacterization of differently expressed genes during the interaction between ‘Fuji’ apples and Botryosphaeria dothidea
dc.typeArtigo de periódico
dc.typeNacional


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