Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts

dc.creatorRibeiro, Aldemir Reginato
dc.creatorNascimento, Vladimir Pinheiro do
dc.creatorCardoso, Martha Oliveira
dc.creatorSantos, Luciana Ruschel dos
dc.creatorRocha, Silvio Luis da Silveira
dc.date2010-04-16T09:11:06Z
dc.date2002
dc.identifier1517-8382
dc.identifierhttp://hdl.handle.net/10183/19662
dc.identifier000372078
dc.descriptionThis study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/ RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased sensitivity and permitted a better isolation of Salmonella. The conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded Salmonella, should be searched.
dc.formatapplication/pdf
dc.languagepor
dc.relationBrazilian journal of microbiology. São Paulo, SP. Vol. 33, n. 4 (out./dez. 2002), p. 339-341
dc.rightsOpen Access
dc.subjectSalmonella : Aves
dc.subjectImmunomagnetic separation
dc.subjectSalmonella
dc.subjectRaw broiler parts
dc.titleUtilization of immunomagnetic separation for detection of Salmonella in raw broiler parts
dc.typeArtigo de periódico
dc.typeNacional


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