A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure
A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure
dc.creator | Hernández, Francisco | |
dc.creator | Akahori, Hiroshi | |
dc.creator | Hosaka, Yasuhiro | |
dc.creator | Akaike, Kaoru | |
dc.creator | Ikegami, Nobuko | |
dc.date | 2017-04-27 | |
dc.date.accessioned | 2023-08-03T18:20:05Z | |
dc.date.available | 2023-08-03T18:20:05Z | |
dc.identifier | https://revistas.ucr.ac.cr/index.php/rbt/article/view/28716 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7900369 | |
dc.description | A new rotary-shadowing process to obtain freeze-drying replicas is described for the analysis of virus ultrastructure, using the inner capsid of human rotavirus as a model. The findings corroborate the icosahedral symmetry with an arrangement pattern of capsomers of T=13L. | en-US |
dc.description | Se describe un método de sombreado rotatorio para preparar réplicas (copias) de viriones estabilizados mediante congelación y secado a baja presión. Esta réplicas permiten el análisis de la ultraestructura viral; empleándose como modelo de estudio la cápside interna de rotavirus humanos. Los hallazgos corroboran el patrón de simetría icosaédrico con un patrón de capsómeros de T=13L, previamente descrito. | es-ES |
dc.format | application/pdf | |
dc.language | eng | |
dc.publisher | Universidad de Costa Rica | en-US |
dc.relation | https://revistas.ucr.ac.cr/index.php/rbt/article/view/28716/28696 | |
dc.source | Revista de Biología Tropical; Vol. 42 No. 2 (1994): Volume 42 – Supplement 2 – August 1994: An electron microscope view of tropical nature: selected studies; 175-178 | en-US |
dc.source | Revista de Biología Tropical; Vol. 42 Núm. 2 (1994): Volumen 42 – Suplemento 2 – Agosto 1994: La naturaleza tropical vista al microscopio electrónico: estudios selectos; 175-178 | es-ES |
dc.source | Revista Biología Tropical; Vol. 42 N.º 2 (1994): Volumen 42 – Suplemento 2 – Agosto 1994: La naturaleza tropical vista al microscopio electrónico: estudios selectos; 175-178 | pt-PT |
dc.source | 2215-2075 | |
dc.source | 0034-7744 | |
dc.source | 10.15517/rbt.v42i2 | |
dc.subject | Rotavirus | en-US |
dc.subject | freeze-drying replica technique | en-US |
dc.subject | ultrastructure | en-US |
dc.title | A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure | en-US |
dc.title | A rotary shadow freeze-drying replica method for the three dimensional view of virus ultrastructure | es-ES |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.type | Article | en-US |