In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)

In vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)

dc.creatorValdez-Melara, Marta
dc.creatorGarcía, Alexander
dc.creatorDelgado, Marlon
dc.creatorGatica-Arias, Andrés M
dc.creatorRamírez-Fonseca, Pilar
dc.date2009-11-01
dc.date.accessioned2023-08-03T18:01:11Z
dc.date.available2023-08-03T18:01:11Z
dc.identifierhttps://revistas.ucr.ac.cr/index.php/rbt/article/view/21279
dc.identifier10.15517/rbt.v57i0.21279
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7898322
dc.descriptionAn efficient and reproducible method for regeneration of commercial and pure lines of tropical butternut squash (Cucurbita moschata) plants via somatic embryogenesis was developed. The influence of genotype, explant source, N6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) concentration on somatic embryogenesis induction was investigated. Friable embryogenic calli was produced from zigotic embryos (53-56%) and cotyledons from seedlings (70%) of C. moschata cv. Sello de Oro cultured on callus induction medium (CIM) supplemented with 0.5 mg/l or 3.5 mg/l 2,4-D. No embryogenic calli was obtained from leaf segments of C. moschata cv. Sello de Oro cultured on CIM supplemented with different concentrations of BAP and 2,4-D and cotyledons from seedlings of C. moschata cv. PVG 04 cultured on CIM with BAP and 2,4,5-T. Embryogenic calli induction was achieved in 75% C. moschata pure lines evaluated and calli percentage frequency range from 5% to 34%. Successful acclimatization of squash in vitro plants was achieved in the greenhouse and in the field. Regenerated plants appeared morphologically normal and set flowers and fruits with seeds that could germinate normally.en-US
dc.descriptionAn efficient and reproducible method for regeneration of commercial and pure lines of tropical butternut squash (Cucurbita moschata) plants via somatic embryogenesis was developed. The influence of genotype, explant source, N6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) concentration on somatic embryogenesis induction was investigated. Friable embryogenic calli was produced from zigotic embryos (53-56%) and cotyledons from seedlings (70%) of C. moschata cv. Sello de Oro cultured on callus induction medium (CIM) supplemented with 0.5 mg/l or 3.5 mg/l 2,4-D. No embryogenic calli was obtained from leaf segments of C. moschata cv. Sello de Oro cultured on CIM supplemented with different concentrations of BAP and 2,4-D and cotyledons from seedlings of C. moschata cv. PVG 04 cultured on CIM with BAP and 2,4,5-T. Embryogenic calli induction was achieved in 75% C. moschata pure lines evaluated and calli percentage frequency range from 5% to 34%. Successful acclimatization of squash in vitro plants was achieved in the greenhouse and in the field. Regenerated plants appeared morphologically normal and set flowers and fruits with seeds that could germinate normally.es-ES
dc.formatapplication/pdf
dc.languageeng
dc.publisherUniversidad de Costa Ricaen-US
dc.relationhttps://revistas.ucr.ac.cr/index.php/rbt/article/view/21279/21503
dc.rightsDerechos de autor 2009 Revista de Biología Tropicales-ES
dc.rightshttp://creativecommons.org/licenses/by/4.0es-ES
dc.sourceRevista de Biología Tropical; Vol. 57 No. S1 (2009): Volume 57 – Supplement 1 – November 2009: 50th anniversary School of Biology, University of Costa Rica; 119–127en-US
dc.sourceRevista de Biología Tropical; Vol. 57 Núm. S1 (2009): Volumen 57 – Suplemento 1 – Noviembre: 50 aniversario Escuela de Biología, Universidad de Costa; 119–127es-ES
dc.sourceRevista Biología Tropical; Vol. 57 N.º S1 (2009): Volumen 57 – Suplemento 1 – Noviembre: 50 aniversario Escuela de Biología, Universidad de Costa; 119–127pt-PT
dc.source2215-2075
dc.source0034-7744
dc.source10.15517/rbt.v57i0
dc.subjectcucurbitsen-US
dc.subjectcucurbita moschataen-US
dc.subjectauxinsen-US
dc.subjectcytokininen-US
dc.subjectexplant sourceen-US
dc.subjectgenotypeen-US
dc.subjectCosta Ricaen-US
dc.subjectcucurbitses-ES
dc.subjectcucurbita moschataes-ES
dc.subjectauxinses-ES
dc.subjectcytokinines-ES
dc.subjectexplant sourcees-ES
dc.subjectgenotypees-ES
dc.subjectCosta Ricaes-ES
dc.titleIn vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)en-US
dc.titleIn vitro plant regeneration system for tropical butternut squash genotypes (Cucurbita moschata)es-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeArticleen-US


Este ítem pertenece a la siguiente institución