Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plant

Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plant

dc.creatorSamantaray, Sanghamitra
dc.creatorMaiti, Satyabrata
dc.date2011-03-01
dc.date.accessioned2023-08-03T17:40:32Z
dc.date.available2023-08-03T17:40:32Z
dc.identifierhttps://revistas.ucr.ac.cr/index.php/rbt/article/view/3210
dc.identifier10.15517/rbt.v59i1.3210
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7896088
dc.descriptionChlorophytum arundinaceum is an important medicinal plant and its tuberous roots are used for various health ailment treatments. It has become an endangered species in the Eastern Ghats, and a rare medicinal herb in India, due to its excessive collection from its natural habitat and its destructive harvesting techniques, coupled with poor seed germination and low vegetative multiplication ratio. In order to contribute to its production systems, an efficient protocol was developed for in vitro clonal propagation through shoot bud culture. For this, multiple shoots were induced from shoot bud explants on Murashige and Skoog's medium supplemented with 2.5-3.0mg/L BAP, 0.01-0.1mg/L NAA and 3% (w/v) sucrose. Inclusion of Adenine Sulphate (25mg/L) in the culture medium improved the frequency of multiple shoot production and recovered the chlorotic symptoms of the leaves. Media having pH 5.9 and 4% sucrose showed significant improvement on shoot bud multiplication and growth. In vitro flowering was observed when the subcultures were carried out for over four months in the same multiplication media. Rooting was readily achieved upon transferring the shoots on to half- strength MS medium supplemented with 0.1mg/L IBA and 2% (w/v) sucrose. Micropropagated plantlets were hardened in the green house, successfully established, and flowered in the field. This method could effectively be applied for the conservation and clonal propagation to meet the demand of planting materials. Rev. Biol. Trop. 59 (1): 435-445. Epub 2011 March 01.en-US
dc.formatapplication/pdf
dc.languageeng
dc.publisherUniversidad de Costa Ricaen-US
dc.relationhttps://revistas.ucr.ac.cr/index.php/rbt/article/view/3210/3116
dc.rightsDerechos de autor 2011 Revista de Biología Tropicales-ES
dc.rightshttp://creativecommons.org/licenses/by/4.0es-ES
dc.sourceRevista de Biología Tropical; Vol. 59 No. 1 (2011): Volume 59 – Regular number 1 – March 2011; 435–445en-US
dc.sourceRevista de Biología Tropical; Vol. 59 Núm. 1 (2011): Volumen 59 – Número regular 1 – Marzo 2011; 435–445es-ES
dc.sourceRevista Biología Tropical; Vol. 59 N.º 1 (2011): Volumen 59 – Número regular 1 – Marzo 2011; 435–445pt-PT
dc.source2215-2075
dc.source0034-7744
dc.source10.15517/rbt.v59i1
dc.subjectddden-US
dc.subjectLiliaceaeen-US
dc.subjectmedicinal planten-US
dc.subjectpHen-US
dc.subjectsucroseen-US
dc.titleFactors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal planten-US
dc.titleFactors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plantes-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeArticleen-US
dc.typeTexten-US


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