| dc.contributor | RUBEN RAMOS GARCIA | |
| dc.creator | Rodrigo Itzamná Becerra Deana | |
| dc.date | 2019 | |
| dc.date.accessioned | 2023-07-25T16:24:33Z | |
| dc.date.available | 2023-07-25T16:24:33Z | |
| dc.identifier | http://inaoe.repositorioinstitucional.mx/jspui/handle/1009/1905 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7807096 | |
| dc.description | Light Sheet Fluorescence Microscopy is a highly efficient technique (in terms of excitation and detection of the signal) to observe, in high resolution (x, y and z), living samples. It works by illuminating the sample with a light sheet, normally produced using a cylindrical lens. The generated fluorescence signal is recorded in a perpendicular way to the light sheet. Until now, LASERS, considered as point sources, had to be used to ensure the production of a thin light sheet. In this project, I present a new way to create a Light Sheet using LEDs or any extended source in general in combination with the use of a Photonic Lantern (PL). This has the advantage that they will produce a more homogenous illumination, have a large bandwidth, produce no speckle or any interference patterns and is not expensive. Thus, in this work, I will introduce the working principle of the Light Sheet Fluorescence Microscope (LSFM), then I will show the different kind of sources of light to generate a Light Sheet, including the use of extended sources. Then I will explain the Photonic Lantern, its constructions, and its characterization. This allows to present, for the first time, the combined use of a PL with an extended source for obtaining high-resolution images in a light sheet microscope. Finally, I will show the use of LEDs and PL as the ideal tool to produce high-resolution images of non-fluorescently labeled samples using elastic scattering as a contrast mechanism. Importantly, this opens up a new range of applications in which fluorescence signal is too weak (for example for fast imaging) or for conditions in which labeling is not possible at all. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Instituto Nacional de Astrofísica, Óptica y Electrónica | |
| dc.relation | citation:Becerra Deana, R. I., (2019), Construction of a light sheet system with novel illumination configurations, Tesis de Maestría, Instituto Nacional de Astrofísica, Óptica y Electrónica. | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0 | |
| dc.subject | info:eu-repo/classification/Inspec/Light sheet | |
| dc.subject | info:eu-repo/classification/Inspec/Photonic lantern | |
| dc.subject | info:eu-repo/classification/Inspec/Light source device | |
| dc.subject | info:eu-repo/classification/cti/1 | |
| dc.subject | info:eu-repo/classification/cti/22 | |
| dc.subject | info:eu-repo/classification/cti/2209 | |
| dc.subject | info:eu-repo/classification/cti/2209 | |
| dc.title | Construction of a light sheet system with novel illumination configurations | |
| dc.type | info:eu-repo/semantics/masterThesis | |
| dc.type | info:eu-repo/semantics/acceptedVersion | |
| dc.audience | students | |
| dc.audience | researchers | |
| dc.audience | generalPublic | |
