| dc.contributor | JULIO CESAR RAMIREZ SAN JUAN | |
| dc.creator | MARIA DEL CARMEN GRADOS LUYANDO | |
| dc.date | 2014-10 | |
| dc.date.accessioned | 2023-07-25T16:21:04Z | |
| dc.date.available | 2023-07-25T16:21:04Z | |
| dc.identifier | http://inaoe.repositorioinstitucional.mx/jspui/handle/1009/179 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7805401 | |
| dc.description | Multimodal confocal mosaic images scan image wide surgical margins
(approximately 1 cm) with sub-cellular resolution and mimic the appearance of
conventional hematoxylin and eosin histopathology (H&E). The goal of this work is
to combine three confocal imaging modalities: acridine orange (AO) fluorescence for
labeling nuclei, eosin fluorescence (Eo) for labeling cytoplasm, and endogenous
reflectance (R) for marking collagen and keratin. Absorption contrast is achieved
by alternating the excitation wavelength: 488 nm (AO fluorescence) and 532 nm
(Eo fluorescence). Scattering contrast is achieved by the reflectance at either or
both of the wavelengths. Superposition and false-coloring of the images from these
three modalities mimics H&E, enabling detection of pancreatic cell carcinomas. The
sum of images Eo + R is false-colored pink to mimic the appearance of eosin, while
the AO mosaic is false-colored purple to mimic the appearance of hematoxylin in
H&E. In this study, mosaics of pancreatic cell carcinomas where processed. The
results suggest that confocal mosaics could be used for pancreatic cancer diagnosis
in the operating room, although the staining process with Eosin may be optimized
as the reflectance mode. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Instituto Nacional de Astrofísica, Óptica y Electrónica | |
| dc.relation | citation:Grados-Luyando M.C. | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0 | |
| dc.subject | info:eu-repo/classification/Cancer/Cancer | |
| dc.subject | info:eu-repo/classification/Microscopía/Microscopy | |
| dc.subject | info:eu-repo/classification/Tintes/Dyes | |
| dc.subject | info:eu-repo/classification/Fluorescencia/Fluorescence | |
| dc.subject | info:eu-repo/classification/cti/1 | |
| dc.subject | info:eu-repo/classification/cti/22 | |
| dc.subject | info:eu-repo/classification/cti/2209 | |
| dc.subject | info:eu-repo/classification/cti/2209 | |
| dc.title | Multimodal microscopy for rapid diagnosis of pancreatic cancer | |
| dc.type | info:eu-repo/semantics/masterThesis | |
| dc.type | info:eu-repo/semantics/acceptedVersion | |
| dc.audience | students | |
| dc.audience | researchers | |
| dc.audience | generalPublic | |
