Metabolic activation of herbicide products by Vicia faba detected in human peripheral lymphocytes using alkaline single cell gel electrophoresis.

dc.creatorMARIA ELENA CALDERON SEGURA
dc.creatorBERTHA MOLINA ALVAREZ
dc.date2007
dc.date.accessioned2023-07-25T16:15:57Z
dc.date.available2023-07-25T16:15:57Z
dc.identifier10.1016/j.tiv.2007.03.002
dc.identifierhttp://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3128
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7805227
dc.descriptionAmetryn and metribuzin S-triazines derivatives and EPTC thiocarbamate are herbicides used extensively in Mexican agriculture, for example in crops such as corn, sugar cane, tomato, wheat, and beans. The present study evaluated the DNA damage and cytotoxic effects of three herbicides after metabolism by Vicia faba roots in human peripheral lymphocytes using akaline single cell gel electrophoresis. Three parameters were scored as indicators of DNA damage: tail length, percentage of cells with DNA damage (with comet), and level DNA damage. The lymphocytes were treated for 2 h with 0.5–5.0 lg/ml ametryn or metribuzin and 1.5–10 lg/ml EPTC. Lymphocytes also were coincubated for 2 h with 20 ll V. faba roots extracts that had been treated for 4 h with 50–500 mg/l of the two triazines or with the thiocarbamate herbicide or with ethanol (3600 mg/l), as positive control. The lymphocytes treated with three pesticides without in vivo metabolic activation by V. faba root did not show significant differences in the mean values bewteen genotoxic parameters compared with negative control. But when human cells were exposed to three herbicides after they had been metabolized the frequency of cell comet, tail length and level DNA damage all increased. At highest concentrations of the three herbicides produced severe DNA damage compared with S10 fraction and negative control. The linear regression analysis of the tail length values of three herbicides indicated that there was genotoxic effect concentration-response relationship with ametryn and ametribuzin but no EPTC. The ethanol induced major increase DNA damage compared with S10 fraction and the three pesticides. There were not effects in cell viability with treatment EPTC and metribuzin wherther or not it had been metabolized. High concentrations of ametryn alone and after it had been metabolized decreased cell viability compared with the negative control. The results demonstrated that the three herbicides needed to be activated by the V. faba root metabolism to produce DNA damage in human peripheral lymphocyte. The alkaline comet technique is a rapid and sensitive assay, to quickly evaluate DNA damage the metabolic activation of herbicide products by V. faba root in human cells in vitro.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPergamon Press
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceToxicol in Vitro 21(6):1143-1154
dc.subjectinfo:eu-repo/classification/cti/3
dc.subjectSupervivencia celular - Efectos de drogas
dc.subjectHerbicidas - Metabolismo
dc.subjectHerbicidas - Toxicidad
dc.subjectLinfocitos - Efectos de drogasas
dc.subjectLinfocitos - Fisiología
dc.subjectMutágenos - Metabolismo
dc.subjectMutágenos - Toxicidad
dc.subjectExtractos vegetales - Farmacología
dc.subjectRaíces de plantas - Metabolismo
dc.subjectTiocarbamatos - Toxicidad
dc.subjectTriazinas - Metabolismo
dc.subjectTriazinas - Toxicidad
dc.subjectVicia faba - Metabolismo
dc.subjectCell Survival - Drug effects
dc.subjectHerbicides - Metabolism
dc.subjectHerbicides - Toxicity
dc.subjectTriazines - Metabolism
dc.subjectTriazines - Toxicity
dc.subjectLymphocytes drug effects
dc.subjectLymphocytes - Physiology
dc.subjectMutagens - Metabolism
dc.subjectMutagens - Toxicity
dc.subjectPlant extracts - Pharmacology
dc.subjectPlant roots - Metabolism
dc.subjectThiocarbamates - Toxicity
dc.subjectVicia faba - Metabolism
dc.subjectAmetryn
dc.subjectMetribuzin
dc.subjectEPTC herbicides
dc.subjectHuman peripheral lymphocytes
dc.subjectIn vivo vicia faba metabolic activation
dc.subjectComet tail length
dc.subjectDNA damaged cells
dc.titleMetabolic activation of herbicide products by Vicia faba detected in human peripheral lymphocytes using alkaline single cell gel electrophoresis.
dc.typeinfo:eu-repo/semantics/article


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