Cytotoxicity of the β- carboline alkaloids harmine and harmaline in human cell assays in vitro01.

dc.creatorJUDITH JIMENEZ GUZMAN
dc.creatorLETICIA RIVERON NEGRETE
dc.creatorFIKRAT ABDULLAEV JAFAROVA
dc.creatorJESUS JAVIER ESPINOSA AGUIRRE
dc.creatorROSARIO RODRIGUEZ ARNAIZ
dc.date2008
dc.date.accessioned2023-07-25T16:15:55Z
dc.date.available2023-07-25T16:15:55Z
dc.identifier10.1016/j.etp.2007.12.003
dc.identifierhttp://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3145
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7805214
dc.descriptionβ-Carboline alkaloids are natural products widely distributed in plants and also found in alcoholic beverages, well-cooked foods and tobacco smoke. Various authors have reported genotoxic activities of several carboline in prokaryotic and eukaryotic cells that have been attributed to their abilities to intercalate into DNA. But studies on the genotoxic and on the cytotoxic potencies in human cells in vitro are not found in the literature. In the present study the toxicities of one full aromatic β-carboline alkaloid (harmine) and one dihydro-β-carboline alkaloid (harmaline) were evaluated by means of two in vitro human cell assays: the cytochalasin-B blocked micronucleus (CBMN) assay and the viability/colony formation assay with four different human cultured non-transformed (CCD18Lu) and transformed (HeLa, C33A and SW480) cells. Neither alkaloid was able to induce micronuclei levels above that of control levels in a wide range of doses tested; although, harmine at the highest concentrations assayed induced apoptotic as well as necrotic cells. Harmine produced a good viability of all cell lines assayed (control and tumor) while harmaline significantly reduced the viability of transformed and non-transformed cell lines in a dose-dependent manner. Harmine displayed a dose-dependent inhibitory effect on cell proliferation against all human carcinoma cells, but the SW480 transformed cell line showed a higher sensitivity. These results suggested that harmine was identified as a useful inhibitor of tumor development. © 2008 Elsevier GmbH. All rights reserved.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceExperimental and Toxicologic Pathology 60(4 - 5 ):381-9
dc.subjectinfo:eu-repo/classification/cti/3
dc.subjectMuerte celular - Efectos de drogas
dc.subjectLínea celular tumoral
dc.subjectProliferación de células - Efectos de drogas
dc.subjectEstimulantes del sistema nervioso central - Toxicidad
dc.subjectHarmalina - Toxicidad
dc.subjectCell death - Drug effects
dc.subjectCell line, tumor
dc.subjectCell proliferation - Drug effects
dc.subjectCentral nervous system stimulants - Toxicity
dc.subjectHarmaline - Toxicity
dc.subjectHarmina - Toxicidad
dc.subjectHarmine - Toxicity
dc.subjectΒ-carbolina alcaloides
dc.subjectCitocalasina B bloqueado ensayo de micronúcleos (CBMN)
dc.subjectViabilidad y ensayo de formación de colonia de células
dc.subjectβ-Carboline alkaloids
dc.subjectHarmaline
dc.subjectCytochalasin-B blocked micronucleus (CBMN) assay
dc.subjectViability and cell colony formation assay
dc.titleCytotoxicity of the β- carboline alkaloids harmine and harmaline in human cell assays in vitro01.
dc.typeinfo:eu-repo/semantics/article


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