Serotonin and 5-hydroxy-indole-acetic acid contents in dorsal raphe and suprachiasmatic nuclei in normal, malnourished and rehabilitated rats under 24 h of sleep deprivation.

dc.creatorALFONSO BENITO ALFARO RODRIGUEZ
dc.creatorRIGOBERTO GONZALEZ PIÑA
dc.creatorANGELICA GONZALEZ MACIEL
dc.creatorEMILIO ARCH TIRADO
dc.date2006
dc.date.accessioned2023-07-25T16:15:50Z
dc.date.available2023-07-25T16:15:50Z
dc.identifier10.1016/j.brainres.2006.06.069
dc.identifierhttp://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3122
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7805185
dc.descriptionIt has been discussed that serotonin (5-HT) could be involved in the effects of sleep deprivation (SD) and/or malnutrition (M) on the sleep-wake cycle. The aim of this work was to study the effects of the M, SD and its interaction on 5-HT and 5-hydroxy-indole-acetic acid (5-HIAA) contents in the dorsal raphe (DR) and the suprachiasmatic nuclei (SCN), two sleep-wake cycle regulators. Forty-eight puppets rats were obtained from mothers fed with low or normal casein diet. They were allocated in 3 groups (n = 16 each): prenatal/postnatal casein malnutrition (6/6%), prenatal casein malnutrition/nutritional casein rehabilitation (6/25%) and prenatal/postnatal casein well-nourished state (25/25%). When rats were 60 days old, 24 animals were exposed to sleep deprivation by means of forced locomotion during 24 h. The remaining 24 were kept under normal conditions of sleep-wake cycle. Then, all animals were sacrificed by decapitation. DR and SCN were dissected and processed to determine the 5-HT and 5-HIAA contents by means of HPLC. It was observed that 6/6% rats showed a 5-HT increase (DR p < 0.011; SCN p < 0.019) as well as in SD (DR p < 0.0008; SCN p < 0.0009) with respect to 25/25% rats. No differences were found in 6/25% rats. Therefore, 5-HIAA decreased significantly in both nuclei in all the groups, notably in M + SD animals (DR p < 0.001; SCN p < 0.001). We conclude that the sleep-wake cycle disruptions produced by chronic M and SD are mediated in part by a synergistic effect on 5-HT in the DR-SCN pathway, perhaps due to a delay in the development of such brain structures. © 2006 Elsevier B.V. All rights reserved.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc/4.0
dc.sourceBrain Research 1110(1):95 - 101
dc.subjectinfo:eu-repo/classification/cti/3
dc.subjectConducta animal - Ratas
dc.subjectCromatografía líquida de alta presión - Métodos - Ratas
dc.subjectÁcido hidroxiindolacético - Metabolismo - Ratas
dc.subjectDesnutrición - Metabolismo - Ratas
dc.subjectDesnutrición - Patología - Ratas
dc.subjectDesnutrición - Rehabilitación - Ratas
dc.subjectNúcleos del rafe - Metabolismo - Ratas
dc.subjectSprague - Dawley - Ratas
dc.subjectSerotonina - Metabolismo - Ratas
dc.subjectPrivación de sueño - Metabolismo - Ratas
dc.subjectNúcleo supraquiasmático - Metabolismo - Ratas
dc.subjectBehavior, animal - Rats
dc.subjectChromatography, high pressure liquid - Methods - Rats
dc.subjectHydroxyindoleacetic acid - Metabolism - Rats
dc.subjectMalnutrition - Metabolism - Rats
dc.subjectMalnutrition - Pathology - Rats
dc.subjectMalnutrition - Rehabilitation - Rats
dc.subjectRaphe nuclei metabolism - Rats
dc.subjectSerotonin - Metabolism - Rats
dc.subjectSleep deprivation - Metabolism - Rats
dc.subjectSuprachiasmatic nucleus - Metabolism - Rats
dc.subjectSerotonina
dc.subjectAcido 5-Hidroxi-Indol-Acético
dc.subjectDorsal núcleos del rafe
dc.subjectNúcleo supraquiasmático
dc.subjectPrivación del sueño, ratas
dc.subjectSerotonin
dc.subject5-Hydroxy-Indole-Acetic Acid
dc.titleSerotonin and 5-hydroxy-indole-acetic acid contents in dorsal raphe and suprachiasmatic nuclei in normal, malnourished and rehabilitated rats under 24 h of sleep deprivation.
dc.typeinfo:eu-repo/semantics/article


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