La citotoxicidad inducida por Casiopeína IIgly a las células HeLa agota los niveles de glutatión reducido y es prevenida por el sulfóxido de dimetilo.

Casiopeína IIgly induced cytotoxicity to HeLa cells depletes the levels of reduced glutathione and is prevented by dimethyl sulfoxide.

dc.creatorFRANCISCO RADAMES ALEMON MEDINA
dc.creatorJOSE LUIS MUÑOZ SANCHEZ
dc.creatorLENA RUIZ AZUARA
dc.creatorMARIA ISABEL GRACIA MORA
dc.date2008
dc.date.accessioned2023-07-25T16:15:44Z
dc.date.available2023-07-25T16:15:44Z
dc.identifier10.1016/j.tiv.2007.11.011
dc.identifierhttp://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3141
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7805139
dc.descriptionThe newly synthesized copper coordination compound Casiopeína IIgly (Cas IIgly) is a promising alternative drug in the treatment of cancer, since it has shown cytotoxicity and genotoxicity in different tumour models. Given its enhanced effects after ascorbic acid-mediated copper reduction, Cas IIgly's activity is thought to be related to oxidative damage. In the present work, oxidized Cas IIgly failed to induce cytosolic oxidative damage in HeLa cells (only 0.9% of the cell population), and in 2.3% of the treated cells when previously reduced, as evaluated through the oxidation of dihydrorhodamine 123 (DHR 123). However, it showed cytotoxicity, since HeLa cells treated with 10-80 microg/mL Cas IIgly proliferated only at 30% of their normal rate, and at 15% when treated with reduced Cas IIgly. This cytotoxicity is strongly abolished in the presence of the hydroxyl scavenger dimethyl sulfoxide. The decrease, from 3994 to 530 nanograms of reduced glutathione (GSH) per million cells after treatment with 80 microg/mL Casiopeína IIgly, indicates that this drug causes the expenditure of this naturally occurring antioxidant. These results altogether suggest that, albeit Cas IIgly induced cytotoxicity is not related to cytosolic DHR 123 oxidation, it may be related to oxidative damage.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPergamon Press
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceToxicology in Vitro 22(3):710-715
dc.subjectinfo:eu-repo/classification/cti/3
dc.subjectAntineoplásicos
dc.subjectAntineoplásicos - Toxicidad
dc.subjectAntioxidantes - Metabolismo
dc.subjectMuerte celular - Efecto de drogas
dc.subjectSupervivencia celular
dc.subjectCitosol - Efectos de drogas
dc.subjectCitosol - Ultrastructura
dc.subjectDimetilsulfóxido - Farmacología
dc.subjectColorantes fluorescentes
dc.subjectGlutation - Metabolismo
dc.subjectAntineoplastic agents - Antagonists & inhibitors
dc.subjectAntineoplastic agents - Toxicity
dc.subjectAntioxidants - Metabolism
dc.subjectCell death - Drug effects
dc.subjectCell survival - Drug effects
dc.subjectCytosol - Drug effects
dc.subjectCytosol - Ultrastructure
dc.subjectDimethyl sulfoxide - Pharmacology
dc.subjectFluorescent dyes
dc.subjectGlutathione - Metabolism
dc.subjectLIgly de la casiopeína
dc.subjectDaño oxidativo
dc.subjectDihydrorhodamine 123 (DHR 123)
dc.subjectGlutatión reducido (GSH)
dc.subjectCasiopeína IIgly
dc.subjectOxidative damage
dc.subjectDihydrorhodamine 123 (DHR 123)
dc.subjectReduced glutathione (GSH)
dc.titleLa citotoxicidad inducida por Casiopeína IIgly a las células HeLa agota los niveles de glutatión reducido y es prevenida por el sulfóxido de dimetilo.
dc.titleCasiopeína IIgly induced cytotoxicity to HeLa cells depletes the levels of reduced glutathione and is prevented by dimethyl sulfoxide.
dc.typeinfo:eu-repo/semantics/article


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