Effect of dichlorvos on hepatic and pancreatic glucokinase activity and gene expression, and on insulin mRNA levels.

dc.creatorJOSE GUILLERMO ROMERO NAVARRO
dc.creatorTERESITA GUADALUPE LOPEZ ACEVES
dc.creatorALBERTO ROJAS OCHOA
dc.creatorMARIA CRISTINA REGINA FERNANDEZ MEJIA
dc.date2006
dc.date.accessioned2023-07-25T16:15:43Z
dc.date.available2023-07-25T16:15:43Z
dc.identifier10.1016/j.lfs.2005.06.010
dc.identifierhttp://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3119
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7805131
dc.descriptionSeveral studies have shown that organophosphate pesticides affect carbohydrate metabolism and produce hyperglycemia. It has been reported that exposure to the organophosphate pesticide dichlorvos affects glucose homeostasis and decreases liver glycogen content. Glucokinase (EC 2.7.1.1) is a tissue-specific enzyme expressed in liver and in pancreatic beta cells that plays a crucial role in glycogen synthesis and glucose homeostasis. In the present study we analyzed the effect of one or three days of dichlorvos administration [20 mg/kg body weight] on the activity and mRNA levels of hepatic and pancreatic glucokinase as well as on insulin mRNA abundance in the rat. We found that the pesticide affects pancreatic and hepatic glucokinase activity and expression differently. In the liver the pesticide decreased the enzyme activity; on the contrary glucokinase mRNA levels were increased. In contrast, pancreatic glucokinase activity as well as mRNA levels were not affected by the treatment. Insulin mRNA levels were not modified by dichlorvos administration. Our results suggest that the decreased activity of hepatic glucokinase may account for the adverse effects of dichlorvos on glucose metabolism. © 2005 Elsevier Inc. All rights reserved.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceLife Sciences 78(9):1015 - 1020
dc.subjectinfo:eu-repo/classification/cti/3
dc.subjectDiclorvos - Farmacología - Ratas
dc.subjectRegulación de la expresión génica - Genética - Ratas
dc.subjectEnzimológica -Efectos de drogas - Ratas
dc.subjectGlucoquinasa - Metabolismo - Ratas
dc.subjectInsecticidas - Farmacología - Ratas
dc.subjectInsulina - Biosíntesis - Ratas
dc.subjectDosificación letal mediana - Ratas
dc.subjectHígado - Efectos de drogas - Ratas
dc.subjectHígado - Enzimología - Ratas
dc.subjectPáncreas - Efectos de drogas - Ratas
dc.subjectPáncreas - Enzimología - Ratas
dc.subjectARN Mensajero - Biosíntesis - Ratas
dc.subjectRatas wistar
dc.subjectDichlorvos - Pharmacology - Rats
dc.subjectGene expression regulation - Genetics - Rats
dc.subjectEnzymologic -Drug effects - rats
dc.subjectGlucokinase - Metabolism - Rats
dc.subjectInsecticides - Pharmacology - Rats
dc.subjectInsulin - Biosynthesis - Rats
dc.subjectLethal dose 50 - Rats
dc.subjectLiver - Drug effects - Rats
dc.subjectLiver - Enzymology - rats
dc.subjectPancreas - Drug effects - Rats
dc.subjectPancreas - Enzymology - Rats
dc.subjectRNA, messenger -Biosynthesis - Rats
dc.subjectRats, wistar
dc.subjectOrganofosforados
dc.subjectPlaguicidas
dc.subjectDiclorvos
dc.subjectGlucoquinasa
dc.subjectInsulina - Ratas
dc.subjectOrganophosphate
dc.subjectPesticides
dc.subjectDichlorvos
dc.subjectGlucokinase
dc.subjectInsulin - rats
dc.titleEffect of dichlorvos on hepatic and pancreatic glucokinase activity and gene expression, and on insulin mRNA levels.
dc.typeinfo:eu-repo/semantics/article


Este ítem pertenece a la siguiente institución