Una estrategia para detectar anomalías cromosómicas en niños con leucemia linfoblástica aguda. /

A Strategy to detect chromosomal abnormalities in children with acute lymphoblastic leukemia.

dc.creatorSILVIA PATRICIA PEREZ VERA
dc.creatorSARA FRIAS VAZQUEZ
dc.creatorJOSE MIGUEL BETANCOURT RULE
dc.creatorMARISA MUJICA SANCHEZ
dc.creatorROBERTO RIVERA LUNA
dc.creatorROCIO ORTIZ LOPEZ
dc.date2004
dc.date.accessioned2023-07-25T16:15:40Z
dc.date.available2023-07-25T16:15:40Z
dc.identifier10.1097/00043426-200405000-00007
dc.identifierhttp://repositorio.pediatria.gob.mx:8180/handle/20.500.12103/3135
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7805107
dc.descriptionConventional cytogenetics (CC) can be used to identify chromosomal abnormalities that are predictors of treatment outcome in acute lymphoblastic leukemia (ALL). The detection of abnormalities in ALL is difficult because low mitotic index and poor-quality metaphases are obtained. Flow cytometry (FC) and fluorescence in situ hybridization (FISH) can be used to detect aneuploidy in any phaseofthecellcycle,increasingthenumberofanalyzablecells.The aim of this study was to develop a strategy combining these methods toimprovethefrequencyofchromosomeabnormalitydetection.One hundred children with newly diagnosed ALL were included. CC and DNA content analysis by FC were performed in all patients. The numericalabnormalitiesidentifiedbybothmethodswerecomparedand patientswereclassifiedasconcordantordiscordant.FISHwasusedto support aneuploidy results in discrepant cases using centromeric probesforthechromosomesmostfrequentlyinvolvedinaneuploidy. CC and FC showed high concordance (86%). Fourteen cases were discrepant: nine showed hypodiploidy and low hyperdiploidy by cytogenetics and five showed high hyperdiploidy by FC. FISH confirmed aneuploidy in 12 cases in which it could be performed. High hyperdiploidywasthemostcommonabnormality;the31casesshowing this aneuploidy were identified by FC. The search for abnormalities must begin by measuring DNA content to detect this aneuploidy, whichisusefultoevaluatethepatient’srisk.However,itisimportant toscreenforstructuralabnormalitiesbyCCormoleculartechniques. This strategy may detect chromosomal abnormalities, optimizing resourcesinlaboratorieswherenotallthescreeningmethodsareavailable.
dc.formatapplication/pdf
dc.languageeng
dc.publisherLippincott, Williams & Wilkins
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceJournal of Pediatric Hematology Oncology 26(5):294 - 300
dc.subjectinfo:eu-repo/classification/cti/3
dc.subjectAberraciones cromosómicas
dc.subjectTécnicas de diagnóstico molecular - Métodos
dc.subjectLeucemia - Linfoma linfoblástico de células precursoras - Genética
dc.subjectChromosome aberrations
dc.subjectMolecular diagnostic techniques - Methods
dc.subjectPrecursor cell lymphoblastic leukemia-Lymphoma - Genetics
dc.subjectAnomalías cromosómicas
dc.subjectLeucemia linfoblástica aguda
dc.subjectContenido de ADN
dc.subjectCitometría de flujo
dc.subjectLa fluorescencia de hibridación in situ
dc.subjectChromosomal abnormalities
dc.subjectAcute lymphoblastic leukemia
dc.subjectDNA content
dc.subjectFlow cytometry
dc.subjectFluorescence in situ hybridization
dc.titleUna estrategia para detectar anomalías cromosómicas en niños con leucemia linfoblástica aguda. /
dc.titleA Strategy to detect chromosomal abnormalities in children with acute lymphoblastic leukemia.
dc.typeinfo:eu-repo/semantics/article


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