Design of species-specific primers for early detection of Kretzschmaria zonata, the causal agent of root and neck tot of teak (Tectona grandis)

dc.creatorAnuar Ahmed Magaña Álvarez
dc.creatorANDRES FELIPE DE JESUS QUIJANO RAMAYO
dc.creatorANGEL NEXTICAPAN GARCEZ
dc.creatorJOSE CIBRIAN TOVAR
dc.creatorSandy Guardia Chalé
dc.creatorYasmín Abril Sánchez Rodríguez
dc.creatorALBERTO CORTES VELAZQUEZ
dc.creatorTERESITA DE JESUS VALENCIA YAH
dc.creatorRodolfo Martín Mex
dc.creatorMARYNOR ELENA ORTEGA RAMIREZ
dc.creatorDaisy de la Caridad Pérez Brito
dc.date2022
dc.date.accessioned2023-07-21T19:20:59Z
dc.date.available2023-07-21T19:20:59Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/2676
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7738214
dc.descriptionKretzschmaria zonata (Lév.) P.M.D. Martin is a fungus that causes root and neck rot in teak (Tectona grandis L.) worldwide. The detection of this fungus in asymptomatic plants is essential for its control but, to date, the disease can only be detected morphologically or by sequencing this fungus’s isolates. The aim of this work was to design, at least, one set of specific PCR primers for a quickly, early and accurate diagnosis of this pathogen. Nineteen isolates of K. zonata from teak plants at different locations in Mexico were obtained. ITS region sequences and two software were used to design the PCR-based primers. All primers obtained were tested on DNA samples from infected teak tissue, K. zonata pure isolates, and other different fungi species (in vitro and in silico). The sensitivity of the primers was evaluated at five different concentrations of target DNA. The primer set KZ-AQ-3F/KZ-AQ-3R was selected for its specificity (amplifies only K. zonata) and sensitivity (1 × 10−5 ng/µL detection). This is the first report of a Kretzschmaria zonata-specific primer set. Tests carried out on asymptomatic teak and artificially inoculated plants with this fungus demonstrated the usefulness of these primers for the accurate detection of K. zonata, and taking early control measures. © 2022 by the authors.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/datasetDOI/https://doi.org/10.3390/f13081175
dc.relationcitation:Magaña-Álvarez, A.; Quijano-Ramayo, A.; Nexticapan-Garcéz, A.; Cibrián-Tovar, J.; Guardia-Chalé, S.; Sánchez-Rodríguez, Y.; Cortés-Velázquez, A.; Valencia-Yah, T.; Martín-Mex, R.; Ortega-Ramírez, M.E.; et al. Design of Species-Specific Primers for Early Detection of Kretzschmaria zonata, the Causal Agent of Root and Neck Rot of Teak (Tectona grandis). Forests 2022, 13, 1175.https://doi.org/10.3390/f13081175
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceForests 2022, 13, 1175.https://doi.org/10.3390/f13081175
dc.subjectinfo:eu-repo/classification/Autores/TECTONA GRANDIS
dc.subjectinfo:eu-repo/classification/Autores/KRETZSCHMARIA ZONATA
dc.subjectinfo:eu-repo/classification/Autores/SPECIFIC PCR PRIMERS
dc.subjectinfo:eu-repo/classification/Autores/TEAK
dc.subjectinfo:eu-repo/classification/Autores/ROOT AND NECK ROT
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2415
dc.subjectinfo:eu-repo/classification/cti/241502
dc.subjectinfo:eu-repo/classification/cti/241502
dc.titleDesign of species-specific primers for early detection of Kretzschmaria zonata, the causal agent of root and neck tot of teak (Tectona grandis)
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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