Bioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus

dc.creatorTania Ortiz López
dc.creatorROCIO DE LOURDES BORGES ARGAEZ
dc.creatorGuadalupe Ayora_Talavera
dc.creatorErnesto Alfonso Canto Ramírez
dc.creatorLISSETH ABIGAIL CETINA MONTEJO
dc.creatorANGEL DE JESUS MAY MAY
dc.creatorFabiola Escalante Erosa
dc.creatorMIRBELLA DEL ROSARIO CACERES FARFAN
dc.date2022
dc.date.accessioned2023-07-21T19:20:58Z
dc.date.available2023-07-21T19:20:58Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/2671
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7738209
dc.descriptionErythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis is a legume tree native to and widely distributed in southeast Mexico, where its branches are used in traditional medicine. An in vitro evaluation of the antiviral activity of extracts and fractions from the leaves, stem bark and roots against two strains of the AH1N1 influenza virus was performed, leading to the identification of bioactive compounds in this medicinal plant. In a cytopathic effect reduction assay, the fractions from the leaves and stem bark were the active elements at the co-treatment level. These were further fractionated based on their hemagglutination inhibition activity. The analysis of spectroscopy data identified a combination of phytosterols (β-sitosterol, stigmasterol and campesterol) in the stem bark active fraction as the main anti-hemagglutinin binding components, while 5-hydroxy-2(2-hydroxy-3,4,5-trimethoxyphenyl)-7-metoxi-4H(chromen-4-ona), which was isolated from the leaf extracts, showed a weak inhibition of viral hemagglutinin. Time of addition experiments demonstrated that the mixture of sterols had a direct effect on viral particle infectivity at the co-treatment level (IC50 = 3.125 µg/mL). This effect was also observed in the virus plaque formation inhibition assay, where the mixture showed 90% inhibition in the first 20 min of co-treatment at the same concentration. Additionally, it was found using qRT-PCR that the NP copy number was reduced by 92.85% after 60 min of co-treatment. These results are the first report of components with anti-hemagglutinin binding activity in the genus Erythrostemon sp. © 2022 by the authors.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/altIdentifier/DOI/doi: 10.3390/molecules27175494
dc.relationcitation:Ortiz-López, T.; Borges-Argáez, R.; Ayora-Talavera, G.; Canto-Ramírez, E.; Cetina-Montejo, L.; May-May, Á.; Escalante-Erosa, F.; Cáceres-Farfán, M. Bioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus. Molecules 2022, 27, 5494. https://doi.org/10.3390/molecules27175494
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceMolecules 2022, 27, 5494. https://doi.org/10.3390/molecules27175494
dc.subjectinfo:eu-repo/classification/Autores/ERYTHROSTEMON YUCATANENSIS
dc.subjectinfo:eu-repo/classification/Autores/INFLUENZA A H1N1 VIRUS
dc.subjectinfo:eu-repo/classification/Autores/ANTI-HEMAGGLUTININ
dc.subjectinfo:eu-repo/classification/Autores/STEROLS
dc.subjectinfo:eu-repo/classification/Autores/FLAVONE
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2403
dc.subjectinfo:eu-repo/classification/cti/230221
dc.subjectinfo:eu-repo/classification/cti/230221
dc.titleBioassay-Guided Fractionation of Erythrostemon yucatanensis (Greenm.) Gagnon & GP Lewis Components with Anti-hemagglutinin Binding Activity against Influenza A/H1N1 Virus
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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