In vitro and in vivo anti-inflammatory properties of Mayan propolis

dc.creatorJORGE FROYLAN XOOL TAMAYO
dc.creatorIVAN HUMBERTO CHAN ZAPATA
dc.creatorVICTOR ERMILO ARANA ARGAEZ
dc.creatorFabiola Elizabeth Villa de la Torre
dc.creatorJULIO CESAR TORRES ROMERO
dc.creatorJESUS ALFREDO ARAUJO LEON
dc.creatorFERNANDO JAVIER AGUILAR AYALA
dc.creatorMARINA EDUVIGES REJON PERAZA
dc.creatorNELLY DEL CARMEN CASTRO LINARES
dc.creatorROSSANA FARIDE VARGAS CORONADO
dc.creatorJUAN VALERIO CAUICH RODRIGUEZ
dc.date2020
dc.date.accessioned2023-07-21T19:19:25Z
dc.date.available2023-07-21T19:19:25Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1885
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7737437
dc.descriptionIntroduction Propolis has been used traditionally for different human diseases and even recently as dental biomaterials because of its antibacterial, antimycotic, and anti-inflammatory properties. However, a proper correlation between in vitro and in vivo anti-inflammatory properties has not been clearly established. Methods The composition of propolis was determined by high-performance liquid chromatography-ultraviolet mass spectrometry (HPLC-UV-MS). Viability of ethanolic propolis solution was evaluated by thiazolyl blue tetrazolium bromide (MTT) assay on murine macrophages. The anti-inflammatory properties were assessed both in vitro through the enzyme-linked immunosorbent assay (ELISA) quantification of various cytokines and in vivo by induced edemas. Results Chemical analysis showed pinocembrin, pinobanksin-3-O-acetate, and pinobanksin-3-O-propionate as the main components of propolis. Macrophage viability was high (106%) when propolis was used up to 50 mu g/mL. ELISA studies showed a reduction in the expression of pro-inflammatory cytokines (IL-1 beta, IL-6, and TNF-alpha) up to 145 pg/mL, 350 pg/mL, and 210 pg/mL, respectively, while the anti-inflammatory cytokines (IL-10 and IL-4) were increased up to 833 pg/mL and 446 pg/mL. Finally, edema was reduced on paw and ear mice by 9% and 22%, respectively. Conclusion Mayan propolis has strong in vitro anti-inflammatory properties without compromising macrophage viability, resulting in a low-to-mild in vivo anti-inflammatory response.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/datasetDOI/10.1177/2058739220935280
dc.relationcitation:Xool-Tamayo, J., Chan-Zapata, I., Arana-Argaez, V. E., Villa-de la Torre, F., Torres-Romero, J. C., Araujo-Leon, J. A., ... & Cauich-Rodríguez, J. V. (2020). In vitro and in vivo anti-inflammatory properties of Mayan propolis. European Journal of Inflammation, 18, 2058739220935280.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceEuropean Journal of Inflammation, 18, 2058739220935280.
dc.subjectinfo:eu-repo/classification/Autores/ANTI-INFLAMMATORY
dc.subjectinfo:eu-repo/classification/Autores/CYTOKINES
dc.subjectinfo:eu-repo/classification/Autores/MACROPHAGES
dc.subjectinfo:eu-repo/classification/Autores/PROPOLIS
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2403
dc.subjectinfo:eu-repo/classification/cti/230222
dc.subjectinfo:eu-repo/classification/cti/230222
dc.titleIn vitro and in vivo anti-inflammatory properties of Mayan propolis
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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