Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs

dc.creatorOSWALDO MARGARITO TORRES CHABLE
dc.creatorLigia Guadalupe Brito Argáez
dc.creatorIgnacio Rodrigo Islas Flores
dc.creatorCLAUDIA VIRGINIA ZARAGOZA VERA
dc.creatorMARITZA ZARAGOZA VERA
dc.creatorGUADALUPE ARJONA JIMENEZ
dc.creatorCARLOS MARCIAL BAAK BAAK
dc.creatorNOHEMI CIGARROA TOLEDO
dc.creatorROBERTO GONZALEZ GARDUÑO
dc.creatorCARLOS IGNACIO MACHAIN WILLIAMS
dc.creatorJULIAN EVERARDO GARCIA REJON
dc.date2020
dc.date.accessioned2023-07-21T19:19:23Z
dc.date.available2023-07-21T19:19:23Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1865
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7737416
dc.descriptionIntroduction: Dirofilaria immitis is a nematode that affects human health in several countries of the world. This study was conducted to examine whether serum samples from the owners of microfilaremic dogs present immunoreactivity to parasite proteins. Methodology: Eight serum samples from the owners of microfilaremic dogs were examined. Total proteins were extracted from adult worms and 12% SDS-PAGE was performed. The gel was electroblotted to a nitrocellulose membrane, and a Western blot (WB) was performed. Reactive bands of 22, 33, 39, 49, and 63 kDa in WB were excised from the gel and analyzed by mass spectrometry (MS). Results: The MS results showed the presence of 10 different proteins of D. immitis recognized by the human serum samples. Conclusions: These results indicate that in endemic areas of D. immitis, owners of infected dogs recognize specific proteins of the parasite, suggesting a possible infection.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/datasetDOI/10.3855/jidc.12711
dc.relationcitation:Torres-Chable OM, Brito-Argaez LG, Islas-Flores IR, Zaragoza-Vera CV, Zaragoza-Vera M, Arjona-Jimenez G, Baak-Baak CM, Cigarroa-Toledo N, Gonzalez-Garduño R, Machain-Williams CI, Garcia-Rejon JE. Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs. J Infect Dev Ctries. 2020 Dec 31;14(12):1442-1447. doi: 10.3855/jidc.12711. PMID: 33378288.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceJ Infect Dev Ctries. 2020 Dec 31;14(12):1442-1447. doi: 10.3855/jidc.12711. PMID: 33378288.
dc.subjectinfo:eu-repo/classification/Autores/DIROFILARIA
dc.subjectinfo:eu-repo/classification/Autores/FILARIASIS
dc.subjectinfo:eu-repo/classification/Autores/IMMUNOREACTIVITY
dc.subjectinfo:eu-repo/classification/Autores/TROPICAL DISEASES
dc.subjectinfo:eu-repo/classification/Autores/ZOONOTIC
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2403
dc.subjectinfo:eu-repo/classification/cti/230221
dc.subjectinfo:eu-repo/classification/cti/230221
dc.titleDirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


Este ítem pertenece a la siguiente institución