Anthelmintic activity of extracts and active compounds from Diospyros anisandra on Ancylostoma caninum, Haemonchus placei and Cyathostomins

dc.creatorGABRIELA JANETT FLOTA BURGOS
dc.creatorJosé Alberto Rosado Aguilar
dc.creatorROGER IVAN RODRIGUEZ VIVAS
dc.creatorROCIO DE LOURDES BORGES ARGAEZ
dc.creatorCINTLI MARTINEZ ORTIZ DE MONTELLANO
dc.creatorMARIA MARCELA GAMBOA ANGULO
dc.date2020
dc.date.accessioned2023-07-21T19:19:21Z
dc.date.available2023-07-21T19:19:21Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1852
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7737404
dc.descriptionThe present study aimed to evaluate the anthelmintic activity of leaf and bark extracts ofDiospyros anisandracollected during different seasons and their major constituents on eggs ofAncylostoma caninum, Haemonchus placei, and cyathostomins. Specifically, the eclosion inhibition of the methanolic extracts of the leaves and bark ofD. anisandracollected during the dry and rainy seasons (600-37.5 mu g/ml) were evaluated in addition to the fractions, sub-fractions (300-37.5 mu g/ml) and active major constituents (150-2.3 mu g/ml). The rainy season bark extract had the highest percentage of eclosion inhibition (PEI) against the evaluated nematodes (>= 90% at 75 mu g/ml) along with high ovicidal activity (90.0 to 93.4% at 75 mu g/ml). The purification of the rainy season bark extract showed that its biological activity came from the non-polarn-hexane fraction (>= 93% at 75 mu g/ml). The bioguided fractionation pointed to sub-fraction 5 as having the highest anthelmintic activity against the three evaluated genera of nematodes (PEI >= 93% at 37.5 mu g/ml). Gas chromatography and mass spectrometry revealed that the major constituent in sub-fraction 5 was plumbagin. Upon evaluation, plumbagin was confirmed to be responsible for the anthelmintic activity ofD. anisandra, with a PEI >= 90% at 2.3 mu g/ml on the three evaluated nematodes. Additionally, the compounds betulin and lupeol in the bark ofD. anisandrawere evaluated but presented low anthelmintic activity (PEI <= 5.3% at 2.3 mu g/ml). In conclusion, the rainy season bark extract ofD. anisandraexerts a high ovicidal activity against the eggs of the three studied nematodes. Plumbagin is the active compound responsible for this activity and represents a potential alternative for the control of different genera of gastrointestinal nematodes given the current scenario of anthelmintic resistance.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/datasetDOI/https://doi.org/10.3389/fvets.2020.565103
dc.relationcitation:Flota-Burgos, G. J., Rosado-Aguilar, J. A., Rodríguez-Vivas, R. I., Borges-Argáez, R., Martínez-Ortiz-de-Montellano, C., & Gamboa-Angulo, M. (2020). Anthelmintic activity of extracts and active compounds from Diospyros anisandra on Ancylostoma caninum, Haemonchus placei and Cyathostomins. Frontiers in Veterinary Science, 7, 675.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceFrontiers in Veterinary Science, 7, 675.
dc.subjectinfo:eu-repo/classification/Autores/ACTIVE COMPOUNDS
dc.subjectinfo:eu-repo/classification/Autores/BIOGUIDED FRACTIONATION
dc.subjectinfo:eu-repo/classification/Autores/GASTROINTESTINAL NEMATODES
dc.subjectinfo:eu-repo/classification/Autores/PLANT EXTRACTS
dc.subjectinfo:eu-repo/classification/Autores/PLUMBBAGIN
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/23
dc.subjectinfo:eu-repo/classification/cti/2306
dc.subjectinfo:eu-repo/classification/cti/230221
dc.subjectinfo:eu-repo/classification/cti/230221
dc.titleAnthelmintic activity of extracts and active compounds from Diospyros anisandra on Ancylostoma caninum, Haemonchus placei and Cyathostomins
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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