Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids

dc.creatorFrancisco Guillén-Chable
dc.creatorULISES RODRIGUEZ CORONA
dc.creatorAlejandro Pereira-Santana
dc.creatorANDREA BAYONA HERNANDEZ
dc.creatorLuis Carlos Rodríguez Zapata
dc.creatorCecilia Aquino Perez
dc.creatorLenka Šebestová
dc.creatorNicolas Vitale
dc.creatorPavel Hozak
dc.creatorEnrique Castaño de la Serna
dc.date2020
dc.date.accessioned2023-07-21T19:19:17Z
dc.date.available2023-07-21T19:19:17Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1822
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7737374
dc.descriptionFibrillarin is a highly conserved nucleolar methyltransferase responsible for ribosomal RNA methylation across evolution from Archaea to humans. It has been reported that fibrillarin is involved in the methylation of histone H2A in nucleoli and other processes, including viral progression, cellular stress, nuclear shape, and cell cycle progression. We show that fibrillarin has an additional activity as a ribonuclease. The activity is affected by phosphoinositides and phosphatidic acid and insensitive to ribonuclease inhibitors. Furthermore, the presence of phosphatidic acid releases the fibrillarin-U3 snoRNA complex. We show that the ribonuclease activity localizes to the GAR (glycine/arginine-rich) domain conserved in a small group of RNA interacting proteins. The introduction of the GAR domain occurred in evolution in the transition from archaea to eukaryotic cells. The interaction of this domain with phospholipids may allow a phase separation of this protein in nucleoli.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/datasetDOI/https://doi.org/10.3390/cells9051143
dc.relationcitation:Guillen-Chable, F., Corona, U. R., Pereira-Santana, A., Bayona, A., Rodríguez-Zapata, L. C., Aquino, C., Šebestová, L., Vitale, N., Hozak, P., & Castano, E. (2020). Fibrillarin Ribonuclease Activity is Dependent on the GAR Domain and Modulated by Phospholipids. Cells, 9(5), 1143. https://doi.org/10.3390/cells9051143
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceCells, 9(5), 1143. https://doi.org/10.3390/cells9051143
dc.subjectinfo:eu-repo/classification/Autores/NUCLEOLUS
dc.subjectinfo:eu-repo/classification/Autores/RIBONUCLEOLAR PARTICLE
dc.subjectinfo:eu-repo/classification/Autores/RRNA
dc.subjectinfo:eu-repo/classification/Autores/FIBRILLARIN
dc.subjectinfo:eu-repo/classification/Autores/PHOSPHOINOSITIDES
dc.subjectinfo:eu-repo/classification/Autores/VIRAL PROGRESSION
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2415
dc.subjectinfo:eu-repo/classification/cti/241502
dc.subjectinfo:eu-repo/classification/cti/241502
dc.titleFibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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