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Molecular identification of selected streptomyces strains isolated from Mexican tropical soils and their anti-Candida activity

dc.creatorDIANA YAMILY ESCALANTE RENDIZ
dc.creatorSUSANA DEL CARMEN DE LA ROSA GARCIA
dc.creatorRAUL TAPIA TUSSELL
dc.creatorJesús Martin
dc.creatorFernando Reyes
dc.creatorFrancisca Vicente Perez
dc.creatorMARIA MARCELA GAMBOA ANGULO
dc.date2019
dc.date.accessioned2023-07-21T19:19:06Z
dc.date.available2023-07-21T19:19:06Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1748
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7737303
dc.descriptionThe increasing incidence of Candida albicans infections and resistance to current antifungal therapies has led to the search for new and more effective antifungal compounds. Actinobacterial species from the Streptomyces genus are recognized as some of the major producers of antimicrobial compounds. Therefore, the aims of this study were: (1) the identification of Streptomyces strains isolated from Mexican tropical acidic soils, (2) the evaluation of their antifungal activity on C. albicans, and (3) the exploration of the presence of polyketide synthase genes in their genome and antifungal secondary metabolites in their extracts. Four actinobacterial strains, isolated from previously unexplored soils with antibacterial antecedents, were selected. These strains were identified as Streptomyces angustmyceticus S6A-03, Streptomyces manipurensis S3A-05 and S3A-09, and Streptomyces parvisporogenes S2A-04, according to their molecular analyses. The ethanol extract of the lyophilized supernatant of S. parvisporogenes displayed the most interesting antifungal activity against C. albicans, with a minimum inhibitory concentration (MIC) of 0.5 mg/mL. Type I polyketide synthase (PKS-I) and non-ribosomal peptide synthase (NRPS) genes were detected in all strains. In addition, type II PKS genes (PKS-II) were also found in S. manipurensis S3A-05 and S. parvisporogenes. LC-UV-HRMS analysis of the active organic extract of S. parvisporogenes indicated the presence of the known antifungal compound carbazomycin G as the major component.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/datasetDOI/https://doi.org/10.3390/ijerph16111913
dc.relationcitation:Escalante-Réndiz, D., de-la-Rosa-García, S., Tapia-Tussell, R., Martín, J., Reyes, F., Vicente, F., & Gamboa-Angulo, M. (2019). Molecular Identification of Selected Streptomyces Strains Isolated from Mexican Tropical Soils and their Anti-Candida Activity. International journal of environmental research and public health, 16(11), 1913. Escalante-Réndiz, D., de-la-Rosa-García, S., Tapia-Tussell, R., Martín, J., Reyes, F., Vicente, F., & Gamboa-Angulo, M. (2019). Molecular Identification of Selected Streptomyces Strains Isolated from Mexican Tropical Soils and their Anti-Candida Activity. Interna
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceInternational journal of environmental research and public health, 16(11), 1913, 2019
dc.subjectinfo:eu-repo/classification/Autores/STREPTOMYCES PARVISPOROGENES
dc.subjectinfo:eu-repo/classification/Autores/ANTIFUNGAL
dc.subjectinfo:eu-repo/classification/Autores/CANDIDA ALBICANS
dc.subjectinfo:eu-repo/classification/Autores/CARBAZOMYCIN G
dc.subjectinfo:eu-repo/classification/Autores/PKS AND NRPS GENES
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2407
dc.subjectinfo:eu-repo/classification/cti/240702
dc.subjectinfo:eu-repo/classification/cti/240702
dc.titleMolecular identification of selected streptomyces strains isolated from Mexican tropical soils and their anti-Candida activity
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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