Effect of inorganic nitrogen source on ammonium assimilation enzymes of Catharanthus roseus plants

dc.creatorMaría de Lourdes Miranda Ham
dc.creatorIRENE GOMEZ
dc.creatorVíctor Manuel Loyola Vargas
dc.date1996
dc.date.accessioned2023-07-21T19:18:56Z
dc.date.available2023-07-21T19:18:56Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1684
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7737244
dc.descriptionThe activities of the enzymes glutamate dehydrogenase (EC 1.4.1.2), glutamine synthetase (EC 6.3.1.2) and glutamate synthase (EC 1.4.1.14) were measured in leaves, roots and leaf explants of Catharanthus roseus grown in different N sources: water (control), 20 mM KNO3, 2 mM NH4Cl and a mixture of the latter two. In the roots, glutamate dehydrogenase activity is fourfold higher than in leaves and it is also higher than the activities of glutamine synthetase and glutamate synthase. The aminative and deaminative GDH activities are regulated differentially depending on the nitrogen source and the tissue studied. Ferredoxin dependent glutamate synthase was detected only in photosynthetic tissues but not in the roots, where the NADH dependent enzyme was found. The N sources substantially modified the total alkaloid content with respect to the control (water): with nitrate producing an increment of 50%, while a mixture of nitrate and ammonium produced a decrease of 45%.
dc.formatapplication/pdf
dc.languageeng
dc.relationcitation:Miranda-Ham, M. D. L., Gómez, I., & Loyola-Vargas, V. M. (1996). Effect of inorganic nitrogen source on ammonium assimilation enzymes of catharanthus roseus plants. Phyton, 58(1-2), 125-133.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourcePhyton, 58(1-2), 125-133, 1996.
dc.subjectinfo:eu-repo/classification/Autores/GLUTAMATE DEHYDROGENASE
dc.subjectinfo:eu-repo/classification/Autores/GLUTAMATE SYNTHASE
dc.subjectinfo:eu-repo/classification/Autores/GLUTAMINE SYNTHETASE
dc.subjectinfo:eu-repo/classification/Autores/NITROGEN SOURCE
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2415
dc.subjectinfo:eu-repo/classification/cti/241502
dc.subjectinfo:eu-repo/classification/cti/241502
dc.titleEffect of inorganic nitrogen source on ammonium assimilation enzymes of Catharanthus roseus plants
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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